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Biosensis/Mouse Insulinlike growth factorbinding protein 1 ELISA Kit (2 Plates)/BEK20312P/1 Ea


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DescriptionThemouseInsulin-likegrowthfactor-bindingprotein1(IGFBP-1)KitisasandwichELISA.Thecaptureantibodyisamonoclonalanti-mouseIGFBP-1antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownIGFBP-1concentrationareaddedtothesewellsandallowedtocomplexwiththeboundIGFBP-1antibody.AbiotinylatedmouseIGFBP-1polyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIGFBP-1presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseIGFBP-1insamplessuchassera,plasma,andcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.Seeproductlabels
OtherNamesIBP-1;IGF-bindingprotein1;IGFBP-1;Igfbp1;Igfbp-1;
AccessionP47876IBP1_MOUSE;
SpecificityMouseInsulin-likegrowthfactor-bindingprotein1
StorageStoreat4°C
KitcomponentsTheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range31.2pg/ml-2,000pg/ml
Sensitivity< 3 pg/ml
KitprotocolClick to download the kit protocolBEK-2031-2P_as_at_Dec2015_protocol.pdf