企业QQ
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| Description | FUNCTION:Receptorforbrain-derivedneurotrophicfactor(BDNF),neurotrophin-3andneurotrophin-4/5butnotnervegrowthfactor(NGF).Involvedinthedevelopmentand/ormaintenanceofthenervoussystem.Thisisatyrosine-proteinkinasereceptor.KnownsubstratesfortheTRKreceptorsareSHC1,PI-3kinase,andPLC-gamma-1.CATALYTICACTIVITY:ATP+a[protein]-L-tyrosine=ADP+a[protein]-L-tyrosinephosphate.SUBUNIT:Existsinadynamicequilibriumbetweenmonomeric(lowaffinity)anddimeric(highaffinity)structures(Bysimilarity).BindsAPS.InteractswithSQSTM1.SUBCELLULARLOCATION:Membrane;single-passtypeImembraneprotein.ALTERNATIVEPRODUCTS:3namedisoformsproducedbyalternativesplicing.Additionalisoformsseemtoexist.TISSUESPECIFICITY:Thedifferentformsaredifferentiallyexpressedinvariouscelltypes.IsoformT2isprimarilyexpressedinneurons.PTM:Ligand-mediatedauto-phosphorylation.SIMILARITY:BelongstotheTyrproteinkinasefamily.Insulinreceptorsubfamily.SIMILARITY:Contains2Ig-likeC2-type(immunoglobulin-like)domains.SIMILARITY:Contains2LRR(leucine-rich)repeats.SIMILARITY:Contains1proteinkinasedomain. |
| Relatedproducts | TrkBcontrolpeptide(aa:23-36),PE-1252-100 |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Asyntheticpeptide(AFPRLEPNSIDPENC)aspartofratmatureTrkBprotein(aa:23-36)conjugatedtoKLH |
| OtherNames | Tropomyosin-relatedkinasereceptor;BDNF/NT-3growthfactorsreceptor;Neurotrophictyrosinekinasereceptortype2;TrkBtyrosinekinase;GP145-TrkB/GP95-TrkB;Trk-B;Ntrk2;Trkb |
| Accession | NTRK2_RAT |
| Producedin | Rabbit |
| Purity | Wholeserum |
| Applications | IHC.Adilutionof1:1000to1:3000isrecommendedforthisapplication.ForWB,a1:500to1:1000dilutionisrecommended.ThesequenceoftheimmunogenandtheconjugateareidenticaltotheoneusedbyYanetal(seetheRefs)andtheantiserumappearstohavesimilarcharacteristics.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | SequencewaschosentoavoidcrossreactivitywithTrkAandTrkC. |
| Cross-reactivity | Thisantiserumrecogniseshuman,ratandmouseTrkB.Crossreactivitywithotherspecieshasnotyetbeentested. |
| Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstability,andat4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | WangT,MartinS,NguyenTH,HarperCB,GormalRS,Martínez-MármolR,KarunanithiS,CoulsonEJ,GlassNR,Cooper-WhiteJJ,vanSwinderenB,MeunierFA.(2016)"FluxofsignallingendosomesundergoingaxonalretrogradetransportisencodedbypresynapticactivityandTrkB."NatCommun.2016Sep.PMID:2768712930(7):12976.Application:IF&structuredilluminationmicroscopy(SIM).Species:Rat,Mouse;fixed4%PFAwith4%sucrose MatusicaDetal(2016)Inhibitionofmotorneurondeathinvitroandinvivobyap75neurotrophinreceptorintracellulardomainfragment.JCellSci.2016Feb1;129(3):517-30.Application:WB. BrockJHetal(2010)Localandremotegrowthfactoreffectsafterprimatespinalcordinjury.JNeurosci.2010Jul21;30(29):9728-37. GruberH.etal(2008)Brain-derivedneurotrophicfactoranditsreceptorinthehumanandthesandratintervertebraldisc.ArthritisResTher.2008;10(4):R82. |
| References | 1.YanQetal.JCompNeurol378:135-57(1997). 2.Stoilov,P.,etal.,Biochem.Biophys.Res.Commun.290(3):1054-1065(2002). 3.Strausberg,R.L.,etal.,Proc.Natl.Acad.Sci.U.S.A.99(26):16899-16903(2002). 4.Nakagawara,A.,etal.,Genomics25(2):538-546(1995). 5.Haniu,M.,etal.,Arch.Biochem.Biophys.322(1):256-264(1995). 6.Allen,S.J.,etal.,Neuroscience60(3):825-834(1994). 7.MizoguchiYetal.,JImmunol.2009Dec15;183(12):7778-86. 8.Spencer-SegalJLetal.JNeurosci.2011May4;31(18):6780-90. 9.NakajimaKetal.Glia.1998Nov;24(3):272-89. |