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Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@biosensis.com| Description | ThehumanNT3KitisasandwichELISA.Thecaptureantibodyisamonoclonalanti-NT3antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.HumantestsamplesandstandardsofknownNT3concentrationareaddedtothesewellsandallowedtocomplexwiththeboundNT3antibody.AbiotinylatedNT3polyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofNT3presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofhumanNT3insamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | BatchNo. | Seeproductlabels | Antigen | NT3isamemberoftheneurotrophinfamily,thatcontrolssurvivalanddifferentiationofvisceralandproprioceptivesensoryneurons.NT3iscloselyrelatedtobothNGFandBDNF.Itmaybeinvolvedinthemaintenanceoftheadultnervoussystem,andmayaffectdevelopmentofneuronsintheembryowhenitisexpressedinhumanplacenta.NT3-deficientmicegeneratedbygenetargetingdisplayseveremovementdefectsofthelimbs.Thematurepeptideofthisproteinisidenticalinallmammalsexaminedincludinghuman,pig,ratandmouse.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Brainandperipheraltissues.SIMILARITY:BelongstotheNGF-betafamily. | OtherNames | NT-3;NTF3;Neurotrophin-3;HDNF;Nervegrowthfactor2;NGF-2; | Accession | P20783NTF3_HUMAN; | Specificity | Human;Mouse;Rat; | Cross-reactivity | Lessthan2.5%crossreactivitywithNT-4.Nodetectablecross-reactivitywithanyothercytokines. | Storage | Storeat4øC | Kitcomponents | TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 15.6pg/ml-1,000pg/ml | Sensitivity | < 2 pg/ml | Kitprotocol | Pleasecontactusatbiospeak@biosensis.comforadetailedprotocolforthisELISAkit. | 新闻动态
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