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Biosensis/Human Matrix metalloproteinase13 ELISA Kit (1 plate)/BEK21801P/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Biosensis/Human Matrix metalloproteinase13 ELISA Kit (1 plate)/BEK21801P/1 Ea


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DescriptionThehumanMatrixmetalloproteinase-13(MMP-13)KitisasandwichELISA.ThecaptureantibodyisapolyclonalhumanMMP-13antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.TestsamplesandstandardsofknownMMP-13concentrationareaddedtothesewellsandallowedtocomplexwiththeboundMMP-13antibody.Abiotinylatedhumanpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofMMP-13presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofhumanMMP-13insamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.Seeproductlabels
OtherNamesCollagenase3;Matrixmetalloproteinase-13;MMP13;MMP-13;
AccessionP45452MMP13_HUMAN;
SpecificityMMP-13
StorageStoreat4øC
KitcomponentsTheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range78pg/ml-5,000pg/ml
Sensitivity< 5 pg/ml
KitprotocolClick to download the kit protocolBEK-2180-1P_as_at_Jun2016_protocol.pdf