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| Description | FUNCTION:Neuropeptidesthatplayasignificantroleintheregulationoffoodintakeandsleep-wakefulness,possiblybycoordinatingthecomplexbehavioralandphysiologicresponsesofthesecomplementaryhomeostaticfunctions.Abroaderroleinthehomeostaticregulationofenergymetabolism,autonomicfunction,hormonalbalanceandtheregulationofbodyfluids,isalsosuggested.Orexin-AbindstobothOX1RandOX2Rwithahighaffinity,whereasorexin-BbindsonlytoOX2Rwithasimilarhighaffinity.SUBCELLULARLOCATION:Endoplasmicreticulum;roughendoplasmicreticulum.Associatedwithperikaryalroughendoplasmicreticulumaswellascytoplasmiclargegranularvesiclesatsynapses.SIMILARITY:Belongstotheorexinfamily. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Asyntheticpeptide(CRLYELLHGAGNHAAGILTL)aspartofBovineOrexinA(aa:14-33)conjugatedtoKLHhasbeenusedastheimmunogen. |
| AntigenLocation | 14-33aa |
| OtherNames | Orexin-A;Hypocretin-1;Hcrt1 |
| Accession | OREX_BOVIN |
| Producedin | Rabbit |
| Purity | Wholeserum |
| Applications | IHC.ThisisasuperbantiserumforimmunohistochemistryonOrexinAcontainingneuronsexhibitingintenselabellingofneuronswithverylowbackground.Adilutionof1:1000to1:2000isrecommendedforthisapplication.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Thespecificityforthisantiserumhasbeenconfirmedbyimmunohistochemistryonratbrainandtheresultsreflectthecurrentliterature. |
| Cross-reactivity | ThisantibodyisknowntoreactwithratOrexinA. |
| Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedIDbetweentheimmunogenusedhereindifferentspeciesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstability,andat4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | 1.KrugerJ.L.etal(2010)Cellularlocationandmajorterminalnetworksoftheorexinergicsysteminthebrainsoffivemicrochiropteranspecies.JChemNeuroanat.2010Nov;40(3):256-62. 2.GaykemaR.P.etal(2009)Lipopolysaccharidechallenge-inducedsuppressionofFosinhypothalamicorexinneurons:theirpotentialroleinsicknessbehavior.BrainBehavImmun.2009Oct;23(7):926-30. 3.LeeH.S.etal(2005)Retrogradestudyofhypocretin-1(orexin-A)projectionstosubdivisionsofthedorsalraphenucleusintherat.BrainRes.2005Oct12;1059(1):35-45. 4.YaoS.T.etal(2005)Waterdeprivationincreasestheexpressionofneuronalnitricoxidesynthase(nNOS)butnotorexin-Ainthelateralhypothalamicareaoftherat.JCompNeurol.2005Sep19;490(2):180-93. |
| References | Nambu,T.etal.(1999)BrainResearch.827:243-60 |