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| Description | Na+/K+ATPaseisanintegralmembraneheterodimer belongingtothefamilyofP-typecationtransportATPases.Theenzymeiscomprisedofalargecatalyticalphasubunitandasmallerbetasubunit.ThealphasubunitcatalyzesthehydrolysisofATPcoupledwiththeexchangeofsodiumandpotassiumionsacrosstheplasmamembrane.Thisactioncreatestheelectrochemicalgradientofsodiumandpotassiumions,providingtheenergyforactivetransportofvariousnutrients. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | SheepkidneyalphasubunitofSodiumPotassiumATPase.Theepitoperecognizedbythisantibodyisbetweenaminoacids646and652. |
| Clone | IMD-66 |
| OtherNames | Na;K-ATPase;ATP1A1;Atpa-1; |
| Accession | P05023TAT1A1_HUMAN |
| Producedin | Mouse |
| Purity | IgG |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof2.0-4.0µg/mlisrecommendedforWB.Boilingtheproteinisnotrecommendedasitcausesaggregation.HumanNa+/K+ATPasesubunitalpha1hasapredictedlengthof1,023aminoacidsandaMWof113kDa.Aconcentrationof4.0-8.0µg/mlisrecommendedtodetectNa+/K+ATPasesubunitalpha1informalinoracetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;chicken; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |