企业QQ
官方微信
Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@biosensis.com| Description | ForquantitativedetectionofmouseHGFincellculturesupernatants,serum,andcitrate,orEDTAtreatedplasmasamplesonlywhenusedasdirected | ThemouseHepatocyteGrowthFactor(HGF)KitisasandwichELISA.ThecaptureantibodyisamonoclonalmouseHGFantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.Standards(expressionsystemforstandard:NSO;Immunogensequence:Q33-R495(α)andV496-L728(β))andtestsamplesareaddedtothewells.Abiotinylatedanti-mouseHGFpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofHGFpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseHGFinsamplessuchassera,plasma,andcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. Range:156pg/mL-10,000pg/mL;Sensitivity<10 pg/mL BatchNo. | Seeproductlabels | Antigen | Standards(expressionsystemforstandard:NSO;Immunogensequence:Q33-R495(α)andV496-L728(β)) | Sequence | Immunogensequence:aminoacids33-495foralphachain,andaminoacids496-728forthebetachainofHGF | OtherNames | Scatterfactor;Hepatopoeitin-A;SF; | Accession | Q08048HGF_MOUSE; | Applications | ELISA | Specificity | NaturalandrecombinantMouseHGF | Cross-reactivity | Thereisnodetectablecross-reactivitywithotherrelevantproteins. | ExpiryDate | Unopenedkitstableat4˚Cfor6months,at-20˚Cfor12months. | Kitcomponents | TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 156pg/mL-10,000pg/mL | Sensitivity | < 10 pg/mL | Kitprotocol | Pleasecontactusatbiospeak@biosensis.comforadetailedprotocolforthisELISAkit. | 新闻动态
行业前沿
技术文章
最新产品
|