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Biosensis/Mouse Ecadherin ELISA Kit (2 plates)/BEK21852P/1 Ea


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DescriptionThemouseE-cadherinKitisasandwichELISA.ThecaptureantibodyisamonoclonalmouseE-cadherinantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.TestsamplesandstandardsofknownE-cadherinconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundE-cadherinantibody.Abiotinylatedpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofE-cadherinpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseE-cadherininsamplessuchassera,plasma,andcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.Seeproductlabels
OtherNamesCadherin-1;ARC-1;Epithelialcadherin;E-cadherin;Uvomorulin;CD324;
AccessionP09803CADH1_MOUSE;
SpecificityE-cadherin
StorageStoreat4°C
KitcomponentsTheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range156pg/ml-10,000pg/ml
Sensitivity< 15pg/ml
KitprotocolClick to download the kit protocolBEK-2185-2P_as_at_May2017.pdf