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Finda1platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@biosensis.com| Description | ThemouseMatrixmetalloproteinase-2(MMP-2)KitisasandwichELISA.ThecaptureantibodyisamouseMMP-2antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownMMP-2concentrationareaddedtothesewellsandallowedtocomplexwiththeboundMMP-2antibody.AbiotinylatedmouseMMP-2antibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofMMP-2presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseMMP-2insamplessuchascellculturesupernatants,serumandheparintreatedplasmasampleswhenusedasdirected.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | BatchNo. | Seeproductlabels | Antigen | Thematrixmetalloproteinases(MMPs)arealargefamilyof zincendopeptidases.AllMMPsaresynthesizedasinactiveproenzymes.TheactivationoftheseproenzymesisacriticalstepthatleadstodegradationofextracellularmatrixcomponentssuchasfibronectinandcollagentypeIII.MMP2isactivatedbyotherMMPssuchasMMP14andMMP16.MMP2isknowntodegradecollagentypesIV,V,VIIandXaswellasgelatintypeI. | OtherNames | 72kDatypeIVCollagenase;EC3.4.24.24;72kDagelatinase;Matrixmetalloproteinase-2;MMP-2;GelatinaseA;TBE-1;MMP2;CLG4A; | Accession | P33434MMP2_MOUSE; | Specificity | MouseMMP-2 | Storage | Storeat4°C | Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 156pg/ml-10,000pg/ml | Sensitivity | < 10 pg/ml | Kitprotocol |  BEK-2068-2P_as_at_Nov2016_protocol.pdf新闻动态
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