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| Description | NestinisamemberoftheclassIVintermediatefilamentproteinfamilywhichisexpressedinneuronalstemcells.ThemolecularweightofhumanNestinasdeterminedbySDS-PAGEmobilityisabout240kDa.Howevertherealmolecularweightisconsiderablylessthanthis,at177kDa,thedisparitybeinglikelyduetothehighlychargedregionoftheC-terminalsegment.Nestinisrelativelypoorlyconservedinproteinsequenceacrossspeciesboundaries,sothatthemouseandhumanproteinshaveanoverallidentityofonly62%.Asaresultantibodiestothehumanproteinoftenfailtorecognizetherodenthomologueandviceversa.HoweverthisantibodystainsbothrodentandhumanNestin.AntibodiestoNestinarewidelyusedtoidentifyneuralstemcells. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Partialsegment(region317-630aa)ofhumanNestinexpressedinE.coli |
| AntigenLocation | 317-630 |
| AntibodyType | Monoclonal |
| Isotype | IgG |
| Clone | 4D11 |
| OtherNames | Nestin;NES; |
| Accession | P48681NEST_HUMAN; |
| Producedin | Mouse,IgG1kappa |
| Purity | Ascitesfluid |
| Applications | WesternBlotting(WB),Immunocytochemistry(IC)andFlowCytometry.SuggesteddilutionforWBis1:1,000-5,000and1:250-500forIC.Use2ug/10^6cellsforFlowCytometry.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Comments | Ready-to-usereagentforin-vitrolaboratoryresearchuseonly. |
| Specificity | Thisantibodyisspecificforthe240kDaNestinproteinbyWBondevelopingratbrain(P18)homogenate.Amuchweakerbandatapprox.90kDamayalsobeseen.Thisissuggestedtobeabreakdownproductofthe240kDaband. |
| AntibodyAgainst | Nestin |
| Cross-reactivity | Human,Rodent |
| BlastURL | Clickhere |
| Form | Lyophilisedfromascitesfluid. |
| Appearance | Whitepowder |
| Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | SchomannT,MezzanotteL,DeGrootJCMJ,RivoltaMN,HendriksSH,FrijnsJHM,HuismanMA(2017)Neuronaldifferentiationofhair-follicle-bulge-derivedstemcellsco-culturedwithmousecochlearmodiolusexplants.PLosOne.12(10):e0187183.Application:ICC/IF;Species:Mouse,Hairfolliclebulge-derivedneuralcrest-derivedstemcells(HFBSCs). GhoCG,SchomannT,deGrootSC,FrijnsJH,RivoltaMN,NeumannMH,HuismanMA.(2015)Isolation,expansionandneuraldifferentiationofstemcellsfromhumanpluckedhair-afurthersteptowardsautologousnerverecovery.CytotechnologyInpress.Application:IF;Species:Human,Hairfolliclebulge-derivedneuralcrest-derivedstemcells(HFBSCs),Keywords:Hairfolliclestemcell,Regeneration,Neuralcrest,Neuron,Glia,Cryopreservation |