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| Description | NT3isamemberoftheneurotrophinfamily,thatcontrolssurvivalanddifferentiationofvisceralandproprioceptivesensoryneurons.NT3iscloselyrelatedtobothNGFandBDNF.Itmaybeinvolvedinthemaintenanceoftheadultnervoussystem,andmayaffectdevelopmentofneuronsintheembryowhenitisexpressedinhumanplacenta.NT3-deficientmicegeneratedbygenetargetingdisplaysevveremovementdefectsofthelimbs.Thematurepeptideofthisproteinisidenticalinallmammalsexaminedincludinghuman,pig,ratandmouse.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Brainandperipheraltissues.SIMILARITY:BelongstotheNGF-betafamily. |
| BatchNo. | Seeproductlabel |
| Unitsize | 500µg |
| Antigen | Asyntheticpeptide(YAEHKSHRGEY)aspartofhuman,mouseandratNT3proteinconjugatedtoBSA |
| OtherNames | Neurotrophin-3;Neurotrophicfactor;HDNF;Nervegrowthfactor2;NGF-2;Ntf3;Ntf-3 |
| Accession | NT3_HUMAN NT3_MOUSE NT3_RAT |
| Producedin | Sheep |
| Purity | ProteinGpurifiedIgG |
| Applications | IHC,ELISA,WB,inhibitionofbiologicalactivity.Aconcentrationof1-10µg/mlisrecommendedforIHC,ELISA,WBandinhibitionofbiologicalactivityinvitro;2-10µg/ml(ED50)forinvivouse.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Acrossreactivityoflessthan0.1%tomouseNGF,recombinanthumanBDNFandNT4/5hasbeenshownbydotblot. |
| Cross-reactivity | Thisantiserumisknowntoreactwithrat,chickenandhumanNT3. |
| Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedIDbetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein500µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstability,andat4ºCwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability. |
| ExpiryDate | 12monthsafterpurchase |
| References | 1.Zouhetal.(1996)Mol.Neurobio.13(3):185-97 2.Rushetal.(1997)Clin.Exp.Pharmacol.24:549-55 3.Tafreshietal.(1998)Neurosci.83(2):373-80 |