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Finda1platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@biosensis.com| Description | TheBiosensisNGFRapidTMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthequantificationofhumanNGFinlessthan3hoursincellculturesupernatants,serum,plasma(citrate)andbrainextractsonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication,inparticularserumandplasmasamples. | ThisELISAkitconsistsofapre-coatedmousemonoclonalanti-NGFcaptureantibody,abiotinylatedanti-NGFdetectionantibodyandhorseradishperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofNGFpresentinsamplesandproteinstandards.AhumanNGFpositivecontrol(QCsample)isprovidedtoassureconsistentassayperformance. ThisNGFELISAkitisdesignedtomeasurehumanNGFandthusemploysarecombinanthumanNGFstandardapprovedbytheWorldHealthOrganization(WHO).DuetoahighdegreeofNGFsequencehomology,theantibodiesusedinthiskitwillalsodetectNGFfromnumerousotherspeciesincludingmouseandrat! TheantibodiesusedinthisELISAkitbindepitopeswithinthematuredomainoftheproteinandthereforecanrecognizeboththepro-andthematureformofNGF.However,internalvalidationdatademonstrates<0.1% cross-reactivity (determined at 25 ng/mL, diluted in assay buffer) with human proNGF protein, suggesting the preferential quantification of mature NGF over full-length human proNGF NotethataccurateNGFquantificationinhumancitrate-plasmarequirestheadditionofHeterophilicAntibodyBlockerBL-005-500providedinthekit,andavailableforpurchaseseparately. Thiskithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. Relatedproducts | BL-005-500,Heterophilicantibodyblocker:SamplediluentadditiveforaccuratequantificationofNGFinhumancitrate-plasma. | BatchNo. | Refertotheproductlabel. | Antigen | FUNCTION:Nervegrowthfactorisimportantforthedevelopmentandmaintenanceofthesympatheticandsensorynervoussystems.Itstimulatesdivisionanddifferentiationofsympatheticandembryonicsensoryneurons.SUBUNIT:Homodimer,associatedbynon-covalentforces.SUBCELLULARLOCATION:Secretedprotein.SIMILARITY:BelongstotheNGF-betafamily. | OtherNames | Beta-nervegrowthfactor;Ngfb | Accession | P01138NGF_HUMAN | Specificity | Human | Cross-reactivity | TheantibodiesusedinthisELISAkitbindepitopeswithinthematuredomainoftheproteinandthereforecanrecognizeboththepro-andthematureformofNGF.However,humanproNGFproteinshows<0.1% cross-reactivity (determined at 25 ng/mL, diluted in assay buffer), suggesting the preferential quantification of mature NGF over full-length human proNGF. | ThisNGFELISAdoesnotcross-reactwithbrainderivedneurotrophicfactor(BDNF),neurotrophin-3(NT-3),NT-4/5,glialcellline-derivedneurotrophicfactor(GDNF)andvascularendothelialgrowthfactor(VEGF165)testedat25ng/mL.DuetoahighdegreeofNGFsequencehomology,theantibodiesusedinthiskitwillalsodetectmatureNGFfromnumerousotherspeciesincludingmouseandrat! Storage | Storeat4°C | ExpiryDate | 12monthsfrompurchase. | Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,QCsample,detectionreagents,heterophilicantibodyblocker,washandsamplebuffersanddetailedprotocols. | Range | 3.9-250pg/mL | Sensitivity | Typicallimitofdetection(LOD)forhumanNGFis2pg/mLdeterminedas150%oftheblankvalue. | Kitprotocol |  BEK-2212_as_at_September2017.pdfMSDS | MSDS_Rapid_ELISA_as_at_Aug2017.pdf新闻动态
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