企业QQ
官方微信
| Description | Lamininisalarge(900kDa)complexextracellularglycoproteinandamajorcomponentofthebasementmembrane.Lamininisthoughttomediatetheattachment,migration,andorganizationofcellsintotissuesduringembryonicdevelopmentbyinteractingwithotherextracellularmatrixcomponents.Lamininiscomposedofthreedifferentpolypeptidechains;alpha,betaandgammawhichareboundtoeachotherbydisulphidebondsintoacross-shapedmolecule.Isoformshavebeendetectedforallthreechains.Thedifferentcombinationsofthealpha,betaandgammachainsgiverisetoatleast15lamininheterotrimers. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Humanlaminin |
| Clone | LAM-26 |
| OtherNames | LAMA;LAMA1; |
| Accession | P25391LAMA1_HUMAN |
| Producedin | Mouse |
| Purity | IgG |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.25-0.5µg/mlisrecommendedforWB.Humanlaminin(subunitalpha-1)hasapredictedlengthof3,075residuesandaMWof337kDa.Aconcentrationof0.5-1.0µg/mlisrecommendedtodetectlaminininformalinfixedandparaffinembeddedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;pig;feline; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |