企业QQ
官方微信
| Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com | |
| Description | The human Interleukin 1 beta (IL-1b) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-1b antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-1b concentration are added to these wells and allowed to complex with the bound IL-1b antibody. A biotinylated anti-human IL-1b antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1b present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-1b in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Batch No. | See product labels |
| Other Names | Interleukin-1 beta; IL-1 beta; Catabolin; IL1B; IL1F2; IL1-beta; |
| Accession | P01584 IL1B_HUMAN; |
| Specificity | Human IL-1b |
| Storage | Store at 4°C |
| Kit components | The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
| Range | 3.9 pg/ml - 250 pg/ml |
| Sensitivity | < 0.15 pg/ml |
| Kit protocol |  BEK-2158-2P_as_at_Sept2016_protocol.pdf |