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| Description | The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Related products | For accurate quantification of GDNF in mouse and human samples, we recommend BEK-2229 and BEK-2222, respectively! While all GDNF RapidTM ELISA kits use the same capture and detection antibodies, the protein standards differ to reflect their use in either human or rodent samples. Note that due to a high degree of sequence homology, GDNF from other species may be quantified. |
| Batch No. | Refer to the product label. |
| Antigen | GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinsons disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily. |
| Other Names | Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor; ATF; GDNF |
| Accession | GDNF_RAT |
| Specificity | Rat. The antibodies used in this kit detect human and mouse GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF RapidTM ELISA kit . All Biosensis GDNF RapidTM ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF RapidTM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF RapidTM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. |
| Cross-reactivity | No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin. |
| Storage | Store at 4°C. |
| Expiry Date | 12 months from purchase. |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plate, protein standards, detection reagents, wash and sample buffers and detailed protocols. |
| Range | 7.8 - 500 pg/mL |
| Sensitivity | Typical limit of detection (LOD) for rrGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10). |
| Kit protocol |  BEK-2230_as_at_Feb2017.pdf |
| MSDS | MSDS_Rapid_ELISA_as_at_Aug2017.pdf |