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| Description | Lipid phosphate phosphohydrolase 3 (LPP3) is a member of the phosphatidic acid phosphatase (PAP) family. LPP3 catalyzes the conversion of phosphatidic acid to diacylglycerol. In addition it hydrolyzes lysophosphatidic acid, ceramide-1-phosphate and sphingosine-1-phosphate (Ref: SWISSPROT). |
| Batch No. | See product label. |
| Unit size | 100 µg |
| Antigen | A synthetic peptide from human LPP3 (179-196 aa) conjugated to KLH. |
| Sequence | YRCRGDDSKVQEARKSFFc-KLH |
| Isotype | Tests as a mouse IgG, with mixed subclasses of IgG1, 2a & 2b |
| Clone | 7H7D3 |
| Other Names | Lipid phosphate phosphohydrolase 3; PAP2-beta; Phosphatidate phosphohydrolase type 2b; Phosphatidic acid phosphatase 2b; PAP-2b; PAP2b; Vascular endothelial growth factor and type I collagen-inducible protein; VCIP; PPAP2B;LPP3 |
| Accession | O14495 LPP3_HUMAN; |
| Produced in | Mouse |
| Molecular Weight | The LPP3 protein has a predicted length of 311 residues and the MW of the monomer is 35 kDa. |
| Applications | Western Blotting (WB), Flow cytometry (FACS) and Immunohistochemistry (IHC). For WB, the recommended concentration is 2-3 µg/mL. For IHC, this antibody has been shown to work on formalin-fixed, paraffin-embedded tissue samples with heat-induced antigen retrieval. The recommended concentration is 0.5-2 µg/mL. For FACS, the recommended concentration is 2.0 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Confirmed by over-expression of human LPP3 cDNA. |
| Cross-reactivity | Human |
| Blast URL | Click here |
| Form | Lyophilised from PBS pH 7.4 |
| Reconstitution | Reconstitute in 100 µL of PBS pH 7.4. Centrifuge to remove any insoluble material. |
| Storage | At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
| Expiry Date | 12 months after purchase |
| Specific References | Mueller PA (2016) PPAP2B expression limits lesion formation in murine models of atherosclerosis. Doctoral Dissertation. Species: Mouse. Application: IHC. Reschen ME, Gaulton KJ, Lin D, Soilleux EJ, Morris AJ, Smyth SS and OCallaghan CA (2015) Lipid-induced epigenomic changes in human macrophages identify a coronary artery disease-associated variant that regulates PPAP2B Expression through Altered C/EBP-beta binding. PLoS Genet. 2015 Apr 2;11(4):e1005061. Species: Human. Application: IHC with heat-induced antigen retrieval. Humtsoe JO, Liu M, Malik AB and Wary KK (2010) Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol Cell Biol. Apr;30(7):1593-606. Species: Human. Application: WB and IHC. |