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| Description | The rat Platelet-derived growth factor (PDGF) Kit is a sandwich ELISA. The capture antibody is a polyclonal rat PDGF-A antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known PDGF concentration are added to these wells and allowed to complex with the bound PDGF antibody. The detection antibody is a biotinylated polyclonal rat PDGF-B antibody so this kit will only quantitate PDGF-AB. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PDGF-AB present in the samples. The purpose of this kit is the in-vitro quantitative determination of human PDGF-AB in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Batch No. | See product labels |
| Other Names | PDGF-2; Platelet-derived growth factor; PDGFB; PDGF2; PDGF-1; PDGFA; PDGF1; RPA1; |
| Accession | P28576 PDGFA_RAT; |
| Specificity | Rat PDGF-AB |
| Storage | Store at 4øC |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
| Range | 31.2 pg/ml - 2,000 pg/ml |
| Sensitivity | < 3 pg/ml |
| Kit protocol | Please contact us at biospeak@biosensis.com for a detailed protocol for this ELISA kit. |