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Finda1platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@biosensis.com| Description | Thehumanp53KitisasandwichELISA.Thecaptureantibodyisapolyclonalhumanp53antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.Testsamplesandstandardsofknownp53concentrationareaddedtothesewellsandallowedtocomplexwiththeboundp53antibody.Abiotinylatedhumanpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.Theintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofp53presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofhumanp53insamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | BatchNo. | Seeproductlabels | Antigen | p53isaDNA-bindingproteincontainingtranscriptionactivation,DNA-bindingandoligomerizationdomains.p53isubiquitouslyexpressedandrespondstoavarietyofcellularstressestoregulatetargetgenesthatinducecellcyclearrest,apoptosis,senescence,DNArepairorchangesinmetabolism.Multiplep53variantsareproducedfromalternativepromotersandalternativesplicing. | OtherNames | Cellulartumorantigenp53;Tumorsuppressorp53;Phosphoproteinp53;NY-CO-13;TP53;P53; | Accession | P04637P53_HUMAN; | Specificity | p53 | Storage | Storeat4°C | SpecificReferences | LiuZHetal.(2014)Prussianblue-dopednanogoldmicrospheresforenzyme-freeelectrocatalyticimmunoassayofp53protein.MicrochimicaActa,Apr2014;181(5):581-8 | Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 156pg/ml-10,000pg/ml | Sensitivity | < 10 pg/ml | Kitprotocol |  BEK-2209-2P_as_at_Jan2012.pdf新闻动态
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