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| Description | Synaptophysinisa38kDaglycoproteinpresentinthemembranesofneuronalpresynapticvesiclesinbrain,spinalcord,retina,vesiclesofadrenalmedulla,neuromuscularjunctionsandendocrinecells.Itisalsoexpressedbyneuroendocrinecells,bothnormalandneoplastic.SynaptophysinisausefulMarkerfortheidentificationofnormalneuroendocrinecellsandneuroendocrineneoplasms. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Ratretinasynaptosome |
| Clone | Syn-58 |
| OtherNames | Syp;p38;SVP38;SP;SYN;SYP1; |
| Accession | P07825SYPH_RAT |
| Producedin | Mouse |
| Purity | IgG |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.Ratsynaptophysinhasapredictedlengthof307residuesandaMWof38kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetectsynaptophysininformalin/acetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Rat |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |