企业QQ
官方微信
| Description | MonoclonalantibodyMC192againsttheratlowaffinitynervegrowthfactorreceptor(p75NTR)isderivedfromthefusionofSp2/0-Ag14myelomacellswithmouseimmunesplenocytes.MC192monoclonalantibodywasoriginallygeneratedbyChandlersetal.p75NTRwasoriginallydiscoveredasalowaffinitynervegrowthfactorreceptor.Lateritwasfoundthatitwasthereceptorforallneurotrophins.Itmediatessignalsofneurotrophinsforneuronalsurvival,apoptosis,neuriteoutgrowthandsynapticplasticity.Recently,ithasbeenrevealedthatp75NTRnotonlyactsasthereceptorforneurotrophinsbutalsothereceptorformanyotherpathologicalligandssuchasprions,rabiesvirusandamyloidbeta.p75NTRalsoactsasaco-receptorforNOGOwhichmediatesinhibitorysignalsofmyelinassociatedprotein.p75NTRishighlyexpressedinanumberofnon-neuronalandneuronalcellsincludingmotorneuronsduringdevelopmentandalsoindamagedneurons.MC192hasapotentialuseastheligandforgenedeliveryintop75NTR-expressingratcellsviaareceptor-mediatedmechanism.FUNCTION:LowaffinityreceptorwhichcanbindtoNGF,BDNF,NT-3,andNT-4.Canmediatecellsurvivalaswellascelldeathofneuralcells.SUBUNIT:Homodimer;disulfide-linked.Interactswithp75NTR-associatedcelldeathexecutor.InteractswithNGFRAP1/BEX3.InteractswithTRAF2,TRAF4,TRAF6,PTPN13andRANBP9.InteractsthroughTRAF6withSQSTM1whichbridgesNGFRtoNTRK1(Bysimilarity). |
| BatchNo. | Seeproductlabel |
| Unitsize | 50µg(1mg/ml) |
| Antigen | Ratp75NTR |
| Isotype | IgG1 |
| Clone | MC192 |
| OtherNames | Low-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR |
| Accession | TNR16_RAT |
| Producedin | Mouse |
| Purity | Immunoglobulin(IgG1)waspurifiedusingProteinGcolumn(AmershamPharmacia),polishedwithSephacryl200HR(AmershamPharmacia)inPBS.TheIgGwasthenconjugatedtoATTO488(ATTOTEC)andpurifiedviagelfiltrationusingaG25finegraingelin10mMTris/50mMNaClsolution. |
| Applications | IF:liveorlightlyfixedcellsortissues(acetoneor4%PFA):2-5ug/mL.Notsuitableforwesternblots;notsuitableforIHonformalinfixedtissues.FACS(20ug/mL)isrecommended,unfixedcells. |
| Specificity | MC192recognizestheextracellulardomainoftheneurotrophinreceptorp75NTRinratanddoesnotreactwithhumanormouseNGFR. |
| SpeciesAgainst | RAT |
| AntibodyAgainst | n-octoglucosidestabilizedproteinscontainingp75receptor,whichwereisolatedfromplasmamembranesofPC-12cells(Chandleretal.,1984). |
| Cross-reactivity | Reactswithrat.DoesnotreactwithmouseorhumanNGFR |
| Form | Liquid |
| Storage | Theantibodyconjugatecanbestoredat4ºCforupto4monthswiththeadditionofappropriateantibacterialagent. |
| SpecificReferences | 1.DaviesA.etal(2010)Thealpha2deltasubunitsofvoltage-gatedcalciumchannelsformGPI-anchoredproteins,aposttranslationalmodificationessentialforfunctionProcNatlAcadSciUSA.Jan26;107(4):1654-9 |
| References | 1.Chandler,C.E.etal(1984)JBiolChem259,6882-6889 2.LagaresAetal(2007)Jofneurosci27(30),7939-7953 |