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| Description | Growth-associated protein 43 (GAP-43) is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is also a major component of neuronal growth cones. GAP-43 binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Phosphorylation of GAP-43 by a protein Kinase C is correlated with certain forms of synaptic plasticity. GAP-43 is used to specifically label injured neurons and to score neuronal regeneration. |
| Batch No. | See product label |
| Unit size | 100 µg |
| Antigen | A synthetic peptide corresponding to a region (216-238) from human Growth-associated protein 43 (GAP-43). To enhance the immunological response, this peptide was coupled to carrier protein BSA. |
| Other Names | Neuromodulin; GAP43; PP46; p57; B-50; F1; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; |
| Accession | P17677 NEUM_HUMAN |
| Produced in | Rabbit |
| Purity | Affinity purified on antigen column |
| Applications | Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5µg/ml is recommended for WB. Human GAP-43 has a predicted length of 238 residues and MW of 25 kDa. A concentration of 0.5-1µg/ml is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. |
| Specificity | The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Cross-reactivity | Human; rat; predicted to react with mouse; |
| Blast it | If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form | Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 added as preservative |
| Reconstitution | Reconstitute in 100 µl of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material. |
| Storage | At least 12 months after purchase at 2 - 4°C (lyophilized formulations).After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles |
| Expiry Date | 12 months after purchase |