企业QQ
官方微信
| Description | Synucleinalphainteractingprotein(Synphilin-1)containsseveralprotein-proteininteractiondomainsandinteractswithalphasynucleininneurons.MutationsofSNCAIPhavebeenlinkedtoParkinsondisease.Theaminoacidsequenceofsynphilin-1sharesahighlevelofidentitywithitshumancounterpart,particularlyinregionscontainingankyrin-likemotifsandthecoiled-coildomain.Expressionpatternofsynphilin-1intissuesissimilarinbothmouseandhuman.Synphilin-1hasanimportantroleintheformationofaggregatesandcytotoxicityinParkinsondiseaseandalsoDorfinmaybeinvolvedinthepathogenicprocessbyubiquitylationofsynphilin-1. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Asyntheticpeptide(SLELNGEKDKDKGRTLQRT)aspartofhumansynphilin-1aconjugatedtoKLHhasbeenusedastheimmunogen. |
| OtherNames | Synphilin-1aprotein;synucleinalphainteractingprotein;Synphilin-1;Synphilin1;SNCAIP |
| Accession | Synphilin-1aprotein_HUMAN |
| Producedin | Rabbit |
| Purity | Wholeserum |
| Applications | IHC.Adilutionof1:1000to1:2000isrecommendedforthisapplication.ThisantiserumwillstainLewybodiesinDorsalraphenucleusofParkinson'sdiseasedhumanbrain.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | AhighlevelofspecificityhasbeenshownforthisantiserumbyIHConhumanbrain. |
| Cross-reactivity | Thisantiserumisknowntoreactwithratandhumansynphilin-1a. |
| Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstability,andat4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| References | 1.Kruger,R.CellTissueRes.318(1),195-199(2004) 2.Lee,G.,etal.J.Biol.Chem.279(8),6834-6839(2004) 3.Tanaka,M.,etal.J.Biol.Chem.279(6),4625-4631(2004) 4.Nagano,Y.,etal.J.Biol.Chem.278(51),51504-51514(2003) 5.Marx,F.P.,etal.Hum.Mol.Genet.12(11),1223-1231(2003) 6.Junn,E.,etal.J.Biol.Chem.277(49),47870-47877(2002) 7.Chung,K.K.,etal.Nat.Med.7(10),1144-1150(2001) 8.Kawamata,H.,etal.J.Neurochem.77(3),929-934(2001) 9.Engelender,S.,etal.Nat.Genet.22(1),110-114(1999) |