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| Description | FUNCTION:Nervegrowthfactorisimportantforthedevelopmentandmaintenanceofthesympatheticandsensorynervoussystems.Itstimulatesdivisionanddifferentiationofsympatheticandembryonicsensoryneurons.SUBUNIT:Homodimer,associatedbynoncovalentforces.SUBCELLULARLOCATION:Secretedprotein.SIMILARITY:BelongstotheNGF-betafamily. |
| BatchNo. | Seeproductlabel |
| Unitsize | 500µg |
| Antigen | NativemousebetaNGFpurifiedfromsubmaxillarysalivarygland(95%puritybyPAGE) |
| OtherNames | Beta-nervegrowthfactor |
| Accession | NGF_MOUSE |
| Producedin | Sheep |
| Purity | ProteinGpurifiedIgG |
| Applications | IHC,1-siteELISA,WB,immunoblot,inhibitionofbiologicalactivity.Aconcentrationof1-3µg/mlisrecommendedforIHC,westernblotandimmunoblot,ELISA,inhibitionofbiologicalactivityinvitro.Useneatforinvivostudiesat2-10µg/ml(ED50).ThisantiserumcompletelyinhibitsneuronalsurvivalandtheoutgrowthactionsofmurineNGFinchickenDRGinvitro.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Acrossreactivityoflessthan1%torecombinanthumanBDNF,NT3,NT4/5byELISAhasbeenshown. |
| Cross-reactivity | Thisantiserumisknowntocrossreactwithmouse,rat,humanandavianNGFbutnotbovineNGF. |
| Blastit | IfyouwouldliketoseethesharedidentitybetweendifferentspeciesorotherproteinsfollowthelinkinAccessionfield,selectthenthesequence(makesurethatyouareselectingthesequencethatyouareinterestedin,asthesequencemaybetheprecursorratherthanthematureproteinforexample)andcopyandpasteitHEREandblast/formatit. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein500µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstability,andat4ºCwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability. |
| ExpiryDate | 12monthsafterpurchse |
| SpecificReferences | FeronFetal(2008)Neurotrophinexpressionintheadultolfactoryepithelium.BrainRes.1196:13-21Application:IHC;Species:Rat |
| GeneralReferences | 1.Ebendal,T.etal(1989)JNeurosciRes22,223-240. 2.Zhou,X.F.etal(1994)JNeurosciMethods54,95-102. 3.Angeletti,P.U.etal(1968)AdvEnzymolRelatAreasMolBiol31,51-75. 4.HesseK.etal.(1997)NeurosciLett.Aug8;231(2):83-6. 5.MiaoJetal.(2012)NeurosciRes.Dec;74(3-4):269-76. |