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Finda1platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@biosensis.com| Description | TheratICAM-1KitisasandwichELISA.ThecaptureantibodyisamousemonoclonalICAM-1antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.RattestsamplesandstandardsofknownICAM-1concentrationareaddedtothesewellsandallowedtocomplexwiththeboundICAM-1antibody.AbiotinylatedratICAM-1polyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofICAM-1presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofratICAM-1/CD54incellculturesupernatants,celllysates,serum,andheparinorEDTAtreatedplasmasamplesonlywhenusedasdirected.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | BatchNo. | Seeproductlabels | Antigen | ThestandardproductusedinthiskitisrecombinantratICAM-1/CD54,consistingaminoacids28-493expressedinandpurifiedfromamammaliancellexpressionsystem.. | OtherNames | ICAM1;ICAM-1;Intercellularadhesionmolecule1;CD54;Icam1;Icam-1; | Accession | Q00238ICAM1_RAT; | Specificity | RatICAM-1 | Storage | Storeat4°C | Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 31.2pg/ml-2,000pg/ml | Sensitivity | < 2 pg/ml | Kitprotocol |  BEK-2025-2P_as_at_Dec2017_protocol.pdf新闻动态
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