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| Description | Ghrelinistheligandforgrowthhormonesecretagoguereceptortype1(GHSR)anduponbindingtothereceptoritinducesthereleaseofgrowthhormonefromthepituitary.Thisligandhasanappetite-stimulatingeffectandisinvolvedingrowthregulation. |
| BatchNo. | Seeproductlabel |
| Unitsize | 50µl |
| Antigen | Asyntheticpeptide(GSSFLSPEHQRVQQRKESKKPPAKLQPR)correspondingtotheaminoacids24-51fromhumanghrelin.Thispeptidewasconjugatedtocarrierproteintoenhancetheimmunologicalresponse. |
| OtherNames | Appetite-regulatinghormone;Growthhormonesecretagogue;Growthhormone-releasingpeptide;Motilin-relatedpeptide;M46protein |
| Accession | GHRL_HUMAN |
| Producedin | Guineapig |
| Purity | Neatserum. |
| Applications | IHC.Aconcentrationof1in3000isrecommendedforIHC.IHCperformedinsheepabomasumdemonstratesintensestainingofcellsandterminals.Nostainingisevidentwhentheprimaryantibodyispre-absorbedwith0.5mg/mlofhumanghrelin.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Specificitywasdemonstratedbyimmunohistochemistry. |
| Cross-reactivity | Thisantibodyisknowntoreactwithsheepandhuman.Otherspecieshavenotyetbeentested. |
| Blastit | Ifyouwouldliketoseethesharedidentitybetweendifferentspeciesorotherproteins,followthelinkintheAccessionfield,selectthesequence(makesurethatyouareselectingthesequencethatyouareinterestedin,asthesequencemaybetheprecursorratherthanthematureproteinforexample)andcopyandpasteitHEREandblast/formatit. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein50µlsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Itisrecommendedthatathawedsampleisstoredat4°Cfornolongerthan2weeks.Allocationofappropriateanti-bacterialagentcanincreaseshelflifebyseveralweeks.Dilutedserumshouldbepreparedasrequired.Longtermstabilityrequiresstorage,preferablyinsmallaliquotsat-20°Corlower.Glycerol(1:1)canbeaddedtoneatserumforadditionalstabilityifintendedusedoesnotpreventthis. |
| ExpiryDate | 12monthsuponreconstitution. |
| References | KojimaM,HosodaH,MatsuoH,KangawaK(2001).Ghrelin;discoveryofthenaturalendogenousligandforthegrowthhormonesecretagoguereceptor.TrendsEndocrinolMetab12pp118-122. |