Exalpha/Rabbit anti Human Toll interacting protein (TOLLIP/TOLIP) (CT)/100 µg/X1824P

BackgroundToll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila.These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). The TLRs act through adaptor molecules to activate various kinases and transcription factors (3) so the organism can respond to potential infection.These adaptor molecules include TOLLIP, MyD88, and TRIF.TOLLIP associates directly with TLR2 and TLR 4, acting as an inhibitor to TLR activation (4).This negative regulation of TLR signaling may serve to limit the production of proinflammatory mediators during infection and inflammation.

Synonyms: TOLLIP (CT): Toll interacting protein

Source

Immunogen: Rabbit polyclonal TOLLIP antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human TOLLIP.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.02% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

ApplicationsTOLLIP antibody can be used for the detection of TOLLIP by Western blot at 1 ug/ml.

Functional Analysis: Western Blotting

Positive Control: Positive Control-Rat Brain LysateLocated in the cytoplasm and phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.5

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References1) Takeda K, Kaisho T, and Akira S.Toll-like receptors.Annu.Rev. Immunol. 2003; 21:335-76.2) Janeway CA Jr. and Medzhitov R.Innate immunerecognition.Annu. Rev. Immunol. 2002; 20:197-216.3) McGettrick AF and O’Neill LAJ.The expanding family ofMyD88-like adaptors in Toll-like receptor signal transduction.Mol. Imm. 2004; 41:577-82.4) Zhang G and Ghosh S.Negative regulation of Toll-likereceptor-mediated signaling by Tollip.J. Biol. Chem. 2002;277:7059-65. 5) UniProtKB/Swiss-Prot entry Q9H0E2. http://cn.expasy.org/uniprot/Q9H0E2, Accessed on February 28, 2007

Protein Reference(s)

Database Name: UniProt

Accession number: Q9H0E2

Species Accession: Human

Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf