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Exalpha/Mouse anti CD14, conjugated to PE/GM-4093/100 Tests188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Exalpha/Mouse anti CD14, conjugated to PE/GM-4093/100 Tests

ProductCode

ProductName

MouseantiCD14,conjugatedtoPE

Size

100Tests

Price(USD)

$346

Background

CD14isaGPI-anchoredmoleculeexpressedbyvirtuallyallhumanmonocytesandmacrophagesandtoalesserdegree-granulocytes.CD14togetherwithToll-likereceptor4andMD-2formstheLPS-receptorcomplexthatrecognizesandsignalsthepresenceofLPS.WhileCD14hasnosignalingstructureitsmainroleseemstobethebindingofLPS.TheMEM18antibodypermitstheidentificationandenumerationofleukocytesusingflowcytometry.MEM18hasbeenalsousedforfunctionalstudiessincethisantibodyblockstheinteractionofLPSwithCD14onmonocytes.Resultsmustbeputwithinthecontextofotherdiagnostictestsaswellastheclinicalhistoryofthepatientbyacertifiedprofessionalbeforefinalinterpretation.Analysesperformedwiththisantibodyshouldbeparalleledbypositiveandnegativecontrols.Ifunexpectedresultsareobtainedwhichcannotbeattributedtodifferencesinlaboratoryprocedures,pleasecontactus

Host

Mouse

Isotype

IgG1

SpeciesReactivity

Human

ProductUsage

TheCD14mAb(cloneMEM18)recognizessurfaceCD14onhumanmonocytesandmacrophagesaswellasonneutrophils.ThesensitivityofMEM18mAbisdeterminedbystainingwell-definedbloodsamplesfromrepresentativedonorswithserial-foldmAbdilutionstoobtainatitrationcurvethatallowsrelatingthemAbconcentrationtothepercentageofstainedcellsandgeometricMFI(meanfluorescenceintensity).Forthispurpose,amAb-concentrationrangeisselectedtoincludeboththesaturationpoint(i.e.themAbdilutionexpectedtobindallepitopesonthetargetcell)andthedetectionthreshold(i.e.themAbdilutionexpectedtorepresenttheleastamountofmAbneededtodetectanidenticalpercentageofcells).Inpractice,50µlofleukocytescontaining10^7cells/mlarestainedwith20µlmAbofvariousdilutionstoobtainatitrationcurveandtoidentifythesaturationpointanddetectionthreshold.Thefinalconcentrationoftheproductisthenadjustedtobeatleast3-foldabovethedetectionthreshold.Inadditionandtocontrollot-to-lotvariation,thegivenlotiscomparedandadjustedtofluorescencestandardswithdefinedintensity.

ProductType

PrimaryAntibodies

Applications

DirectImmunofluorescence,IndirectImmunofluorescence,FlowCytometry

ProductFormulation

PBSpH7.2,1%BSA,0.05%NaN3

StorageConditions

Nordic-Mubiomonoclonalantibodyreagentscontainoptimalconcentrationsofaffinity-purifiedantibody.ForstABIlityreasonsthismonoclonalantibodysolutioncontainssodiumazide.Thesereagentsshouldbestoredat2-8
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