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ARBOR ASSAYS/Green Fluorescent Protein (GFP)/L003-25UG/25UG

    • Purifiedtohomogeneityfromthejellyfish,Aequoreavictoria
    • RecombinantwildtypeproteinexpressedinE.coli
    • Finalhighresolutiongelfiltrationpurified

    Greenfluorescentprotein(GFP),a27kDaproteinderivedfromthejellyfishAequoreavictoria,emitsgreenlight(emissionpeak509nm)whenexcitedbybluelight(excitationpeak395nm).GFPhasbecomeaninvaluabletoolincellBIOLOGyresearch,sinceitsintrinsicfluorescencecanbevisualizedinlivingcells.GFPfluorescenceisstableunderfixationconditionsandsuitableforavarietyofapplications.GFPhasbeenwidelyusedasareporterforgeneexpression,enablingresearcherstovisualizeandlocalizeGFP-taggedproteinswithinlivingcellswithouttheneedforchemicalstaining.OtherapplicationsofGFPincludeassessmentofprotein-proteininteractionsthroughtheyeasttwohybridsystemandmeasurementofdistancebetweenproteinsthroughfluorescenceenergytransfer(FRET)protocols.GFPisusedtomeasuresinglecellmetastasisandsuccessfulproliferationofstemcells.

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