Rabbit MonoclonalRevmab/Anti-Histone H3.3 G34W Rabbit Monoclonal Antibody, Clone RM263; Histone H3.3 G34W Mutant (50ug)/31-1145-00/1 Ea
RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-...
ProductDetailsProductNameAnti-HistoneH3.3G34WRabbitMonoclonalAntibody[RM263]ProductDescriptionRabbitmonoclonaltoHistoneH3.3G34W;HistoneH3.3G34WMutantCatalogNo.31-1145-00CloneNameRM263ProductDataSheetAnti-HistoneH3.3G34WMutantRabbitMonoclonalAntibodyDataSheetRM263SpecificityRM263reactstotheHistoneH3.3G34Wmutant.NocrossreactivitywithwildtypeHistoneH3.3.ImmunogenApeptidecorrespondingtoHistoneH3.3G34WmutantApplicationWesternBlot
ELISA
Immunohistochemistry
Immunocytochemistry
ChromatinIP
SpeciesReactivityAllConjugateNoneIntendedUseResearchUseOnly.PropertiesFormLiquidStorageInstructionsStoreat-20.0°CStABIlityStablefor1Yearat-20.0°CfromdateofreceiptStorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazideVolume/Size50ugConcentration1.0mg/mLPurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatantCloneTypeMonoclonalIsotypeRabbitIgGUsageWesternBlot:0.5ug/mL–2ug/mL;
ELISA:0.5ug/mL-2ug/mL;
Immunohistochemistry(IHC):0.5–5ug/mL;
Immunocytochemistry(ICC):0.5–5ug/mL.
ReferencesforHistoneH3.3G34WMutantantibody[RM263]YamamotoH.etal.DiagnosticutilityofhistoneH3.3G34W,G34R,andG34Vmutant-specificantibodiesforgiantcelltumorsofbone.HumanPathology(2017)10.1016/j.humpath.2017.11.020IHC;Human.Readmore(PubMed:29241742)
Kato,I.etalGiantCellTumoursofBoneTreatedwithDenosumab:Histologic,Immunohistochemical,andH3F3AMutationAnalysesHistopathology10.1111/his.13448.(2017).IHC-P;Human.IF;HumanReadmore(PubMed:29206281)
LukeJ.etal.H3F3AmutationingiantcelltumouroftheboneisdetectedbyimmunohistochemistryusingamonoclonalantibodyagainsttheG34WmutatedsiteofthehistoneH3.3variant.Histopathology10.1111/his.13190.(2017).IHC-P;Human.Readmore(PubMed:28211081)
AmaryF.etal.H3F3A(Histone3.3)G34WImmunohistochemistry:AReliableMarkerDefiningBenignandMalignantGiantCellTumorofBone.AMJSurgPathol.10.1097/PAS.0000000000000859.(2017).IHC-P;Human.Readmore(PubMed:28505000)
.ProductImages
WesternBlotanalysisofcelllysatespreparedfrom
293T, transfected with a DNA construct encoding
G34W mutant or wild type proteins of Histone H3.3,
using anti-Histone H3.3 G34W clone RM263.
Immunohistochemicalstainingofformalinfixedand
paraffin embedded 293T cells transfected with a DNA
construct encoding Histone H3.3 G34W mutant or wild
type, stained with anti-Histone H3.3 G34W clone RM263.
ImmunohistochemicalstainingofGiantCellBone
Tumor (GCBT) tissue sections using Anti-Histone
H3.3 G34W antibody RM263.
ImagecourtesyofAdrienneFlanagan,Department
of Histopathology, RONH, Stanmore, Middlesex, UK
ImmunohistochemicalstainingofGiantCellBone
Tumor (GCBT) tissue sections using Anti-Histone
H3.3 G34W antibody RM263.
ImagecourtesyofAdrienneFlanagan,Department
of Histopathology, RONH, Stanmore, Middlesex, UK
RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.
