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| ProductName: | b-AP15 |
|---|---|
| AlsoKnownAs: | N/A |
| CatalogNo.: | F2100 |
| Size: | 2mg |
| MolecularWeight: | 419.4Da |
| Species: | N/A |
| Source: | Synthetic |
| Stock: | Powder |
| Concentration: | N/A |
| QualityAssurance: | >98%byHPLCandNMR |
| Storage: | EligIBLeforroomtemperatureshipping.Storeat-80°Cuponreceiving;avoidmultiplefreeze-thawcyclesafterdissolvinginDMSO. |
| PDFDataSheet: | DownloadPDFdatasheet |
| NCBIRefSeq: | N/A |
| Image(s): | No |
| ShippingMethod: | Roomtemperatureshipping |
| References: | 1.D’AcryP,etal.(2011)Nat.Med.17.1636.Detailsb-AP15isaspecificinhibitorofthedeubiquitinatingenzymesUch37andUsp14ofthe26Sproteasome.Itblocksthedeubiquitinatingactivityofthe26Sproteasome.Treatingcellswithb-AP15causestheaccumulationofpolyubiquitinatedproteins,inhibitionofproteasomaldegradation,upregulationofapoptoticmarkersandcelldeath.
Images (Clickimagetoenlarge)  EqualamountofwholecelllysatespreparedfromDMSO(lane1)or2μMb-AP15(lane2)-treatedHeLacellswereseparatedbySDS-PAGEandimmunoblottedwithananti-Ubantibody.HEK293TCellsweretreatedwithDMSOorb-AP15for5hours.
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