Predicted Species Reactivity:PigApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Linearity:| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 106-115% (n = 20) | 101-109% (n = 20) | 83-93% (n = 20) | 102-112% (n = 20) |
| EDTA plasma(n=5) | 103-113% (n = 20) | 88-97% (n = 20) | 113-123% (n = 20) | 100-110% (n = 20) |
| heparin plasma(n=5) | 95-105% (n = 20) | 109-119% (n = 20) | 86-96% (n = 20) | 94-104% (n = 20) |
Kit Principle:Aviva Systems Biology FGF1 ELISA Kit (Pig) (OKEH07237) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGF1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGF1 captured in well.Kit Range:78 - 5000pg/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=4.6% (n="">Mean Inter-assay CV%: <=7.0% (n="">Kit Component:| Component | Amount |
|---|
| FGF1 Microplate | 96 Wells (12 x 8 Well strips) |
| FGF1 Lyophilized Standard | 2 |
| 100X Biotinylated FGF1 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.:::Subcellular Location: Secreted Cytoplasm Cytoplasm Cell cortex Cytoplasm Cytosol Nucleus Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59 (By similarity).:::Unigene: Ssc.16246SMR: P20002String: 9823.ENSSSCP00000019575:::Pfam: PF00167:::Pfam: PF00167Reconstitution and Storage:Store as indicated in product manual.Sensitivity:19pg/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Pig Fibroblast growth factor 1Assay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:FGF1Alias Symbols:FGF-1, Acidic fibroblast growth factor, aFGF, Endothelial cell growth factor, ECGF, Heparin-binding growth factor 1, HBGF-1Protein Name:Fibroblast growth factor 1Swissprot Id:P20002
