产品资料

avivasysbio/PDIA4 ELISA Kit (Bovine) (OKEH07611)/96 Wells/OKEH07611

Predicted Species Reactivity:

Bovine

Application:

ELISA

Kit Detection Method:

Colorimetric, OD450 nm

Kit Principle:

Aviva Systems Biology PDIA4 ELISA Kit (Bovine) (OKEH07611) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDIA4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDIA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDIA4 captured in well.

Kit Recovery:

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114% (n = 20)

Kit Reproducibility:

Mean Intra-assay CV%: <=5.0% (n="">Mean Inter-assay CV%: <=9.4% (n="">

Kit Component:

Component	Amount
PDIA4 Microplate	96 Wells (12 x 8 Well strips)
PDIA4 Lyophilized Standard	2
100X Biotinylated PDIA4 Detector Antibody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Sample Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

:::

Subcellular Location: Endoplasmic reticulum lumen Melanosome

:::

Unigene: Bt.23128SMR: Q29RV1String: 9913.ENSBTAP00000022782

:::

KEGG: bta:415110Pfam: PF00085

:::

KEGG: bta:415110Pfam: PF00085

Reconstitution and Storage:

Store as indicated in product manual.

Datasheets/Manuals:

Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Specificity:

Natural and recombinant Bovine Protein disulfide-isomerase A4

Assay Info:

Assay Methodology: Quantitative Sandwich ELISA

Gene Symbol:

PDIA4

Official Gene Full Name:

protein disulfide isomerase family A member 4

NCBI Gene Id:

415110

Protein Name:

protein disulfide-isomerase A4

Swissprot Id:

Q29RV1

Protein Accession #:

NP_001039344.1

Nucleotide Accession #:

NM_001045879.2

Tissue Tool:

Find tissues and cell lines supported by DNA array analysis to express PDIA4.

RNA Seq:

Find tissues and cell lines supported by RNA-seq analysis to express PDIA4.

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avivasysbio/PDIA4 ELISA Kit (Bovine) (OKEH07611)/96 Wells/OKEH07611