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Sino Biological/Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein (S1 Subunit, His Tag)/1/40021-V08H

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Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein (S1 Subunit, His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human coronavirus HKU1 (isolate N1) (HCoV-HKU1) spike protein (S1 Subunit) (YP_173238.1) (Met 1-Arg 760) was expressed with a polyhistidine tag at the C-terminus.
Accession#
YP_173238.1
Expressed Host
HEK293 Cells
Species
HCoV-HKU1
Predicted N Terminal
Gly 16
Molecule Mass
The recombinant human coronavirus HKU1 (isolate N1) (HCoV-HKU1) spike protein (S1 Subunit, His Tag) consists of 756 amino acids with a predicted molecular mass of 85.8 kDa. As a result of high glycosylation, the apparent molecular mass of the recombinant protein is approximately 130-140 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein (S1 Subunit, His Tag): Images

HCoV CoV Spike glycoprotein Protein 11003

Coronavirus spike Background Information

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term "peplomer" is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It"s been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
References
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

SARS Coronavirus spike Proteins

  • SARS-CoV Spike S2-His Recombinant Protein40150-V08B3
  • SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)40150-V08B1
  • SARS-CoV Spike/RBD Protein (RBD, His Tag)40150-V08B2
  • SARS-CoV Spike/RBD Protein (RBD, mFc Tag)40150-V05H
  • SARS-CoV Spike/S1 Protein (S1 Subunit, mFc Tag)40150-V05H1
  • SARS-CoV Spike S1+S2 ECD-His Recombinant Protein (S577A, Isolate Tor2)40634-V08B
  • SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag), Biotinylated40150-V08B1-B
  • SARS-CoV Spike/RBD Protein (RBD, rFc Tag)40150-V31B2
  • SARS-CoV Spike/RBD Protein (RBD, His Tag), Biotinylated40150-V08B2-B

2019-nCoV Coronavirus spike Proteins

  • SARS-CoV-2 (2019-nCoV) Spike RBD(A348S)-His Recombinant Protein40592-V08H25
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombinant Protein, Biotinylated40592-V08B-B
  • SARS-CoV-2 (2019-nCoV) Spike RBD(A520S)-His Recombinant Protein40592-V08H20
  • SARS-CoV-2 (2019-nCoV) Spike RBD(Y508H)-His Recombinant Protein40592-V08H12
  • SARS-CoV-2 (2019-nCoV) Spike S2-mFc Recombinant Protein40590-V05B
  • SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein40589-V08H4
  • SARS-CoV-2 (2019-nCoV) Spike RBD(K378R)-His Recombinant Protein40592-V08H33
  • SARS-CoV-2 (2019-nCoV) Spike RBD(Q409E)-His Recombinant Protein40592-V08H34
  • SARS-CoV-2 (2019-nCoV) Spike RBD(A372T)-His Recombinant Protein40592-V08H36
  • SARS-CoV-2 (2019-nCoV) Spike RBD(E406Q)-His Recombinant Protein40592-V08H40
  • SARS-CoV-2 (2019-nCoV) Spike RBD(K378N)-His Recombinant Protein40592-V08H42
  • SARS-CoV-2 (2019-nCoV) Spike RBD(F338L)-His Recombinant Protein40592-V08H26
  • SARS-CoV-2 (2019-nCoV) Spike RBD(A522V)-His Recombinant Protein40592-V08H16
  • SARS-CoV-2 (2019-nCoV) Spike RBD(F456L)-His Recombinant Protein40592-V08H71
  • SARS-CoV-2 (2019-nCoV) Spike RBD(F486S)-His Recombinant Protein40592-V08H74
  • SARS-CoV-2 (2019-nCoV) Spike RBD(P337S)-His Recombinant Protein40592-V08H62
  • SARS-CoV-2 (2019-nCoV) Spike RBD(K444R)-His Recombinant Protein40592-V08H54
  • SARS-CoV-2 (2019-nCoV) Spike S1 NTD-His & AVI Recombinant Protein, Biotinylated40591-V49H-B
  • SARS-CoV-2 (2019-nCoV) Spike S1 NTD-His & AVI Recombinant Protein40591-V49H
  • SARS-CoV-2 (2019-nCoV) Spike RBD(N487R)-His Recombinant Protein40592-V08H75
  • SARS-CoV-2 (2019-nCoV) Spike RBD(E484Q)-His Recombinant Protein40592-V08H81
  • SARS-CoV-2 (2019-nCoV) Spike RBD(F490L)-His Recombinant Protein40592-V08H83
  • SARS-CoV-2 (2019-nCoV) Spike S1(L18F, D614G)-His Recombinant Protein40591-V08H6
  • SARS-CoV-2 (2019-nCoV) Spike RBD(L455F)-His Recombinant Protein40592-V08H68
  • SARS-CoV-2 (2019-nCoV) Spike S1(A222V, D614G)-His Recombinant Protein40591-V08H4
  • SARS-CoV-2 (2019-nCoV) Spike RBD(G446S)-His Recombinant Protein40592-V08H76
  • SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein40591-V08B1
  • SARS-CoV-2 (2019-nCoV) Spike RBD(P521S)-His Recombinant Protein40592-V08H29
  • SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified)40591-V08H
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombinant Protein40592-V08B
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His (N354D) Recombinant Protein40592-V08H2
  • SARS-CoV-2 (2019-nCoV) Spike RBD(I472V)-His Recombinant Protein40592-V08H35
  • SARS-CoV-2 (2019-nCoV) Spike S1(D614G),ECD-Fc Recombinant Protein40591-V02H3
  • SARS-CoV-2 (2019-nCoV) Spike RBD(S477R)-His Recombinant Protein40592-V08H64
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His (K458R) Recombinant Protein40592-V08H7
  • SARS-CoV-2 (2019-nCoV) Spike RBD(L452R)-His Recombinant Protein40592-V08H28
  • SARS-CoV-2 (2019-nCoV) Spike RBD(T478I)-His Recombinant Protein40592-V08H30
  • SARS-CoV-2 (2019-nCoV) Spike RBD(V483I)-His Recombinant Protein40592-V08H31
  • SARS-CoV-2 (2019-nCoV) Spike RBD(S477N)-His Recombinant Protein40592-V08H46
  • SARS-CoV-2 (2019-nCoV) Spike RBD(T385A)-His Recombinant Protein40592-V08H47
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His (A435S) Recombinant Protein40592-V08H4
  • SARS-CoV-2 (2019-nCoV) Spike RBD(N481D)-His Recombinant Protein40592-V08H70
  • SARS-CoV-2 (2019-nCoV) Spike RBD(D405V, Q414A)-His Recombinant Protein40592-V08H22
  • SARS-CoV-2 (2019-nCoV) Spike RBD(A352S)-His Recombinant Protein40592-V08H58
  • SARS-CoV-2 (2019-nCoV) Spike S1 NTD-Fc & AVI Recombinant Protein, Biotinylated40591-V41H-B
  • SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, N501Y, D614G)-His Recombinant Protein40591-V08H7
  • SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y453F, D614G)-His Recombinant Protein40591-V08H8
  • SARS-CoV-2 (2019-nCoV) Spike RBD(E484K)-His Recombinant Protein40592-V08H84
  • SARS-CoV-2 (2019-nCoV) Spike S2 ECD(708S-1207E)-His Recombinant Protein40590-V08H1
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His (F342L) Recombinant Protein40592-V08H6
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His (V367F) Recombinant Protein40592-V08H1
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His (V483A) Recombinant Protein40592-V08H5
  • SARS-CoV-2 (2019-nCoV) Spike RBD(F377L)-His Recombinant Protein40592-V08H27
  • SARS-CoV-2 (2019-nCoV) Spike RBD(S477I)-His Recombinant Protein40592-V08H45
  • SARS-CoV-2 (2019-nCoV) Spike S2 ECD-Fc Recombinant Protein40590-V02H
  • SARS-CoV-2 (2019-nCoV) Spike RBD(E471Q)-His Recombinant Protein40592-V08H56
  • SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (HPLC-verified)40592-V05H
  • SARS-CoV-2 (2019-nCoV) Spike RBD(A520V)-His Recombinant Protein40592-V08H39
  • SARS-CoV-2 (2019-nCoV) Spike RBD(A344S)-His Recombinant Protein40592-V08H37
  • SARS-CoV-2 (2019-nCoV) Spike RBD(N439K)-His Recombinant Protein40592-V08H14
  • SARS-CoV-2 (2019-nCoV) Spike RBD(N440K)-His Recombinant Protein40592-V08H55
  • SARS-CoV-2 (2019-nCoV) Spike RBD(K417N)-His Recombinant Protein40592-V08H59
  • SARS-CoV-2 (2019-nCoV) Spike RBD(P521R)-His Recombinant Protein40592-V08H63
  • SARS-CoV-2 (2019-nCoV) Spike RBD-AVI & His Recombinant Protein, Biotinylated40592-V27B-B
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His (W436R) Recombinant Protein40592-V08H9
  • SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His Recombinant Protein40592-V08H80
  • SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein40592-V08H82
  • SARS-CoV-2 (2019-nCoV) Spike S1(K417N, E484K, N501Y, D614G)-His Recombinant Protein40591-V08H10
  • SARS-CoV-2 (2019-nCoV) Spike RBD-rFc Recombinant Protein40592-V31H
  • SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein, HPLC-verified40591-V08H3
  • SARS-CoV-2 (2019-nCoV) Spike S2 ECD-His Recombinant Protein40590-V08B
  • SARS-CoV-2 (2019-nCoV) Spike S2 ECD-His Recombinant Protein, Biotinylated40590-V08B-B
  • SARS-CoV-2 (2019-nCoV) Spike RBD(Q414E)-His Recombinant Protein40592-V08H23
  • SARS-CoV-2 (2019-nCoV) Spike RBD-mFc (V367F) Recombinant Protein40592-V05H1
  • SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (D614G)-His Recombinant Protein40589-V08B4
  • SARS-CoV-2 (2019-nCoV) Spike RBD(V341I)-His Recombinant Protein40592-V08H11
  • SARS-CoV-2 (2019-nCoV) Spike RBD(V503F)-His Recombinant Protein40592-V08H15
  • SARS-CoV-2 (2019-nCoV) Spike RBD(V395I)-His Recombinant Protein40592-V08H49
  • SARS-CoV-2 (2019-nCoV) Spike RBD(G482S)-His Recombinant Protein40592-V08H53
  • SARS-CoV-2 (2019-nCoV) Spike RBD(K458Q)-His Recombinant Protein40592-V08H69
  • SARS-CoV-2 (2019-nCoV) Spike RBD(F456E)-His Recombinant Protein40592-V08H73
  • SARS-CoV-2 (2019-nCoV) Spike RBD(F490S)-His Recombinant Protein40592-V08H41
  • SARS-CoV-2 (2019-nCoV) Spike RBD(S359N)-His Recombinant Protein40592-V08H32
  • SARS-CoV-2 (2019-nCoV) Spike RBD(P384L)-His Recombinant Protein40592-V08H24
  • SARS-CoV-2 (2019-nCoV) Spike RBD(T393P)-His Recombinant Protein40592-V08H48
  • SARS-CoV-2 (2019-nCoV) Spike S1 NTD-Fc & AVI Recombinant Protein40591-V41H
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombinant Protein40592-V08H
  • SARS-CoV-2 (2019-nCoV) Spike S1-Fc Recombinant Protein40591-V02H
  • SARS-CoV-2 (2019-nCoV) Spike RBD(A475V)-His Recombinant Protein40592-V08H50
  • SARS-CoV-2 (2019-nCoV) Spike RBD(V445F)-His Recombinant Protein40592-V08H79
  • SARS-CoV-2 (2019-nCoV) Spike S1(N234Q)-His Recombinant Protein40591-V08H11
  • SARS-CoV-2 (2019-nCoV) Spike S1(T20N, D614G)-His Recombinant Protein40591-V08H5
  • SARS-CoV-2 (2019-nCoV) Spike RBD Recombinant Protein40592-VNAH
  • SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombinant Protein,Biotinylated40592-V08H-B
  • SARS-CoV-2 (2019-nCoV) Spike RBD(R408I)-His Recombinant Protein40592-V08H10
  • SARS-CoV-2 (2019-nCoV) Spike RBD(A522S)-His Recombinant Protein40592-V08H21
  • SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein, Biotinylated40591-V08H-B
  • SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein40589-V08B1
  • SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein, Biotinylated40589-V08B1-B
  • SARS-CoV-2 (2019-nCoV) Spike RBD-Fc Recombinant Protein (HPLC-verified)40592-V02H
  • SARS-CoV-2 (2019-nCoV) Spike RBD(G446V)-His Recombinant Protein40592-V08H51
  • SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombinant Protein40592-V08H8
  • SARS-CoV-2 (2019-nCoV) Spike RBD(N370S)-His Recombinant Protein40592-V08H43
  • SARS-CoV-2 (2019-nCoV) Spike RBD(Q414R)-His Recombinant Protein40592-V08H44
  • SARS-CoV-2 (2019-nCoV) Spike RBD(S494P)-His Recombinant Protein40592-V08H18
  • SARS-CoV-2 (2019-nCoV) Spike RBD(A372S)-His Recombinant Protein40592-V08H19
  • SARS-CoV-2 (2019-nCoV) Spike RBD(Y505C)-His Recombinant Protein40592-V08H72
  • SARS-CoV-2 (2019-nCoV) Spike RBD(G485S)-His Recombinant Protein40592-V08H52
  • SARS-CoV-2 (2019-nCoV) Spike RBD(P479S)-His Recombinant Protein40592-V08H57
  • SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-AVI & His Recombinant Protein, Biotinylated40589-V27B-B
  • SARS-CoV-2 (2019-nCoV) Spike S1-mFc Recombinant Protein40591-V05H1
  • SARS-CoV-2 (2019-nCoV) Spike RBD(P499R)-His Recombinant Protein40592-V08H78
  • SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His Recombinant Protein40591-V08H12
  • SARS-CoV-2 (2019-nCoV) Spike S1-AVI & His Recombinant Protein, Biotinylated40591-V27H-B
  • SARS-CoV-2 (2019-nCoV) Spike RBD-AVI & His Recombinant Protein, Biotinylated40592-V27H-B

HCoV-OC43 Coronavirus spike Proteins

  • Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECD, His Tag)40607-V08B
  • Human coronavirus (HCoV-OC43) Spike S2 Protein (ECD, His Tag)40607-V08B1
  • Human coronavirus (HCoV-OC43) Spike S1 Protein (His Tag)40607-V08H1

MERS-CoV Coronavirus spike Proteins

  • MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag)40069-V08B1
  • MERS-CoV Spike/RBD Protein fragment (RBD, aa 367-606, His Tag)40071-V08B1
  • MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)40069-V08B
  • MERS-CoV Spike/RBD Protein fragment (RBD, aa 367-606, His Tag), Biotinylated40071-V08B1-B
  • MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag), HPLC-verifiedHPLC-40069-V08H
  • MERS-CoV Spike/RBD Protein fragment (RBD, aa 367-606, rFc Tag)40071-V31B1
  • MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag)40069-V08H
  • MERS-CoV Spike/S2 Protein (S2 Subunit, aa 726-1296, His Tag)40070-V08B

HCoV-HKU1 Coronavirus spike Proteins

  • Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) Spike/S1 Protein (S1 Subunit, His Tag)40602-V08H
  • Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) Spike Protein (S1+S2 ECD, His Tag)40606-V08B

HCoV-NL63 Coronavirus spike Proteins

  • Human coronavirus (HCoV-NL63) Spike Protein (S1+S2 ECD, His Tag)40604-V08B
  • Human coronavirus (HCoV-NL63) Spike/S1 Protein (S1 Subunit, His Tag)40600-V08H

HCoV-229E Coronavirus spike Proteins

  • Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECD, His Tag)40605-V08B
  • Human coronavirus (HCoV-229E) Spike/S1 Protein (S1 Subunit, His Tag)40601-V08H
  • Comparative Serological Study for the Prevalence of Anti-MERS Coronavirus Antibodies in High- and Low-Risk Groups in Qatar
    Author
    Al Kahlout, R;Nasrallah, G;Farag, E;Wang, L;Lattwein, E;Müller, M;El Zowalaty, M;Al Romaihi, H;Graham, B;Al Thani, A;Yassine, H;
    Year
    2019
    Journal
    Journal of Immunology Research
    Application
    ELISA
    PubMed ID: 30906787FoldedExpand
  • Analysis of SARS-CoV-2 Antibodies in COVID-19 Convalescent Blood using a Coronavirus Antigen Microarray
    Author
    De Assis, RR;Jain, A;Nakajima, R;Jasinskas, A;
    Year
    2020
    Journal
    BioRxiv
    FoldedExpand
  • Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray
    Author
    Khan, S;Nakajima, R;Jain, A;De Assis, RR;Jasinskas, A;
    Year
    2020
    Journal
    BioRxiv
    FoldedExpand

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