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Sino Biological/DKK3 ELISA Kit, Mouse/1/KIT50247

Description
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Citations

DKK3 ELISA Kit, Mouse: General Information

Product name

DKK3 ELISA Kit, Mouse

Assay type

Solid Phase Sandwich ELISA (quantitative)

Conjugate

HRP

Sample type

Serum

Sensitivity

4.62 pg/mL

Assay range

12.5-800 pg/mL

Specificity

Recognizes both recombinant and natural Mouse DKK3

Precision

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
N	20	20	20	20	20	20
Mean(pg/mL)	132.00	243.00	481.00	132.00	262.00	495.00
SD	7.98	7.82	12.03	4.51	19.00	29.02
CV(%)	6.0%	3.2%	2.5%	3.4%	7.3%	5.9%

	Intra-assay Precision
Sample	1	2	3
N	20	20	20
Mean(pg/mL)	132.00	243.00	481.00
SD	7.98	7.82	12.03
CV(%)	6.0%	3.2%	2.5%
	Inter-assay Precision
Sample	1	2	3
N	20	20	20
Mean(pg/mL)	132.00	262.00	495.00
SD	4.51	19.00	29.02
CV(%)	3.4%	7.3%	5.9%

Recovery

The recovery of Mouse DKK3 spiked to different levels throughout the range of the assay in related matrices was evaluated.

Sample	Average % Recovery	Range
serum (n=3)	83	82 -84%

Sample
serum (n=3)
Average % Recovery
83
Range
82 -84%

Linearity

Serum

	Recovery of detected
1:2	95%
1:4	103%
1:8	99%
1:16	101%

Materials provided

1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution

Product overview

This DKK3 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse DKK3 protein in Serum . It contains recombinant Mouse DKK3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Shipping

This ELISA Kit is shipped at ambient temperature.

Storage

Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA

DKK3 ELISA Kit, Mouse: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

DKK3 ELISA Kit, Mouse: Synonyms

AW061014 ELISA Kit, Mouse; C87148 ELISA Kit, Mouse

DKK3 Background Information

DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.

Full Name

dickkopf WNT signaling pathway inhibitor 3

Related Pathways

Canonical Wnt Pathway

References

Yue W, et al., 2008. Downregulation of Dkk3 activates beta-catenin/TCF-4 signaling in lung cancer. Carcinogenesis, 29 (1): 84-92.
Ding Z, et al., 2009. Promoter methylation and mRNA expression of DKK3 and WIF-1 in hepatocellular carcinoma. World J Gastroenterol, 15 (21):2595-601.

DKK3 Matched ELISA Antibody Pair Set,Human
Assay Range(pg/ml):39.1-2500
SEKA10203
DKK3 Matched ELISA Antibody Pair Set,Mouse
Assay Range(pg/ml):15.63-1000
SEK50247

DKK3 (Dickkopf 3) Alters Atherosclerotic Plaque Phenotype Involving Vascular Progenitor and Fibroblast Differentiation Into Smooth Muscle Cells
Author
Karamariti, E;Zhai, C;Yu, B;Lei, Q;Wang, Z;Potter, CMF;Wong, MM;Simpson, RML;Zhang, Z;Wang, X;Del Barco Barrantes, I;Niehrs, C;Kong, D;Zhao, Q;Zhang, Y;Hu, Y;Zhang, C;Xu, Q;
Year
2017
Journal
Arterioscler. Thromb. Vasc. Biol.
Application
ELISA (serum)
PubMed ID: 29284609FoldedExpand

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Sino Biological/DKK3 ELISA Kit, Mouse/1/KIT50247

Description

Related Products

Citations

DKK3 ELISA Kit, Mouse: General Information

DKK3 ELISA Kit, Mouse: Images

DKK3 ELISA Kit, Mouse: Synonyms

DKK3 Background Information

Citation List

If you couldn"t find the specific citation, you can: