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Sino Biological/Anti-SerpinB3 Antibody, Rabbit Polyclonal/1/13218-T52

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Anti-SerpinB3 Antibody, Rabbit Polyclonal General Information

Product name

Anti-SerpinB3 Antibody, Rabbit Polyclonal

Validated applications

WB,ELISA,IP

Species reactivity

Reacts with: Human

Specificity

Human SerpinB3

Immunogen

Recombinant Human SERPINB3 / SCCA-1 protein (Catalog#13218-H07B)

Preparation

Produced in rabbits immunized with purified, recombinant Human SERPINB3 / SCCA-1 (rh SERPINB3 / SCCA-1; Catalog#13218-H07B; AAB20405.1; Asn2-Pro390). SERPINB3 / SCCA-1 specific IgG was purified by Human SERPINB3 / SCCA-1 affinity chromatography.

Source

Polyclonal Rabbit IgG

Purification

Protein A & Antigen Affinity

Formulation

0.2 μm filtered solution in PBS

Conjugate

Unconjugated

Form

Liquid

Shipping

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-SerpinB3 Antibody, Rabbit Polyclonal Validated Applications

Application	Dilution
WB	1:500-1:1000
ELISA	1:25000-1:50000
IP	0.2-1 μL/mg of lysate

Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-SerpinB3 Antibody, Rabbit Polyclonal Images

Human SERPINB3 / SCCA-1 Western blot (WB) 13542

Anti-SERPINB3(2-390) rabbit polyclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800)at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:45 kDa

Observed band size:42 kDa

(We are unsure as to the identity of these extra bands.)

Human SERPINB3 / SCCA-1 Immunoprecipitation(IP) 15298

SERPINB3 was immunoprecipitated using:

Lane A:0.5 mg A431 Whole Cell Lysate

0.5 µL anti-SERPINB3 rabbit polyclonal antibody and 15 μl of50 %Protein G agarose.

Primary antibody:

Anti-SERPINB3 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 43 kDa

Observed band size: 43 kDa

Anti-SerpinB3 Antibody, Rabbit Polyclonal: Synonyms

Anti-HsT1196 Antibody; Anti-SCC Antibody; Anti-SCCA-1 Antibody; Anti-SCCA-PD Antibody; Anti-SCCA1 Antibody; Anti-T4-A Antibody

SerpinB3 Background Information

SERPINB3, also known as SCCA-1, belongs to the serpin family. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases. SERPINB3 is expressed in some hepatocellular carcinoma (at protein level). Its expression is closely related to cellular differentiation in both normal and malignant squamous cells. It seems to also be secreted in plasma by cancerous cells but at a low level. SERPINB3 significantly attenuates apoptosis by contrasting cytochrome c release from the mitochondria and by antichemotactic effect for NK cells. It may act as a protease inhibitor to modulate the host immune response against tumor cells and may be involved in the malignant behavior of squamous cell carcinoma cells.

Full Name

serpin peptidase inhibitor, clade B (ovalbumin), member 3

Research Areas

Serine Proteases and Regulators

References

Schneider SS, et al. (1995) A serine proteinase inhibitor locus at 18q21.3 contains a tandem duplication of the human squamous cell carcinoma antigen gene. Proc Natl Acad Sci. 92(8):3147-51.
Suminami Y, et al.. (1992) Squamous cell carcinoma antigen is a new member of the serine protease inhibitors. Biochem Biophys Res Commun. 181(1):51-8.
Mino-Miyagawa N, et al. (1990) Tumor-antigen 4. Its immunohistochemical distribution and tissue and serum concentrations in squamous cell carcinoma of the lung and esophagus. Cancer. 66(7):1505-12.

Anti-SerpinB3 Antibody
【Rabbit】【Monoclonal】【IP】
13218-R018
Anti-SerpinB3 Antibody
【Rabbit】【Polyclonal】【ELISA】
13218-RP01
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【Mouse】【Monoclonal】【WB,ELISA】
13218-MM13
Anti-SerpinB3 Antibody
【Rabbit】【Polyclonal】【IHC-P,ICC/IF】
204174-T10

The kinetic profile and clinical implication of SCC-Ag in squamous cervical cancer patients undergoing radical hysterectomy using the Simoa assay: a prospective observational study
Author
Ye, S;Sun, X;Kang, B;Wu, F;Zheng, Z;Xiang, L;Lesénéchal, M;Heskia, F;Liang, J;Yang, H;
Year
2020
Journal
BMC Cancer
PubMed ID: 32085736FoldedExpand

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Sino Biological/Anti-SerpinB3 Antibody, Rabbit Polyclonal/1/13218-T52

Description

Related Products

Citations

Antibody Production Service