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Sino Biological/TNF-alpha Protein, Human, Recombinant (hFc Tag)/1/10602-H01H

Description
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Citations
Protein Expression Service

TNF-alpha Protein, Human, Recombinant (hFc Tag): Product Information

Purity

> 90 % as determined by SDS-PAGE.

Endotoxin

< 1.0="" eu="" per="" μg="" protein="" as="" determined="" by="" the="" lal="">

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the human TNF (NP_000585.2) (Val77-Leu233) was expressed with the Fc region of human IgG1 at the N-terminus.

Accession#

NP_000585.2

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Glu

Molecule Mass

The recombinant human TNF consists of 417 amino acids and predicts a molecular mass of 45.8 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNF-alpha Protein, Human, Recombinant (hFc Tag): Images

Human TNF-alpha/TNFA/TNFSF2 Protein 8958

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED₅₀ for this effect is typically 0.5-3ng/mL.

TNF-alpha Protein, Human, Recombinant (hFc Tag): Synonyms

DIF Protein, Human; TNF-alpha Protein, Human; TNFA Protein, Human; TNFSF2 Protein, Human

TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody
Cancer Immunotherapy
Immune Checkpoint
Immunotherapy
Targeted Therapy

Full Name

tumor necrosis factor

Research Areas

Cancer Drug Targets
Neuroinflammation

Related Pathways

Death Receptor Signaling
NF-kB (NFkB) Pathway
IL17 signaling pathway

References

Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.

Human TNF-alpha Proteins

TNF-alpha Protein, Human, Recombinant10602-HNAE
TNF-alpha Protein, Human, Recombinant, Biotinylated10602-HNAE-B
TNF-alpha Protein, Human, RecombinantGMP-10602-HNAE

Mouse TNF-alpha Proteins

TNF-alpha Protein, Mouse, Recombinant (ECD)50349-MNAE
TNF-alpha Protein, Mouse, Recombinant, Biotinylated50349-MNAE-B

Rat TNF-alpha Proteins

TNF-alpha Protein, Rat, Recombinant80045-RNAE

Cynomolgus TNF-alpha Proteins

TNF-alpha Protein, Cynomolgus, Recombinant90330-CNAE
TNF-alpha Protein, Cynomolgus, Recombinant, Biotinylated90330-CNAE-B

Canine TNF-alpha Proteins

TNF-alpha Protein, Canine, Recombinant70003-DNAE

Ferret TNF-alpha Proteins

TNF-alpha Protein, Ferret, Recombinant60002-FNAE

Cynomolgus, Rhesus TNF-alpha Proteins

TNF-alpha Protein, Cynomolgus, Rhesus, Recombinant90018-CNAE

EFab domain substitution as a solution to the light-chain pairing problem of bispecific antibodies
Author
Cooke, HA;Arndt, J;Quan, C;Shapiro, RI;Wen, D;Foley, S;Vecchi, MM;Preyer, M;
Year
2018
Journal
MAbs
Application
SPR
PubMed ID: 30215570FoldedExpand

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Sino Biological/TNF-alpha Protein, Human, Recombinant (hFc Tag)/1/10602-H01H

Description

Related Products

Citations

Protein Expression Service

TNF-alpha Protein, Human, Recombinant (hFc Tag): Product Information

TNF-alpha Protein, Human, Recombinant (hFc Tag): Images