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Sino Biological/BAMBI ELISA Kit, Human/1/KIT10890

Description
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Citations

BAMBI ELISA Kit, Human: General Information

Product name

BAMBI ELISA Kit, Human

Assay type

Solid Phase Sandwich ELISA (quantitative)

Conjugate

HRP

Sample type

Lysate

Sensitivity

7.06 pg/mL

Assay range

12.5-800 pg/mL

Specificity

Recognizes both recombinant and natural Human BAMBI

Precision

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
N	20	20	20	20	20	20
Mean(pg/mL)	107.58	243.46	483.73	109.11	232.49	476.92
SD	4.24	8.01	11.99	4.11	12.29	44.18
CV(%)	3.9%	3.3%	2.5%	3.8%	5.3%	9.3%

	Intra-assay Precision
Sample	1	2	3
N	20	20	20
Mean(pg/mL)	107.58	243.46	483.73
SD	4.24	8.01	11.99
CV(%)	3.9%	3.3%	2.5%
	Inter-assay Precision
Sample	1	2	3
N	20	20	20
Mean(pg/mL)	109.11	232.49	476.92
SD	4.11	12.29	44.18
CV(%)	3.8%	5.3%	9.3%

Recovery

The recovery of Human BAMBI spiked to different levels throughout the range of the assay in related matrices was evaluated.

Sample	Average % Recovery	Range
supernatant (n=3)	72	71 -74%

Sample
supernatant (n=3)
Average % Recovery
72
Range
71 -74%

Linearity

Supernatant

	Recovery of detected
1:2	114%
1:4	117%
1:8	108%
1:16	92%

Materials provided

1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution

Product overview

This BAMBI ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human BAMBI protein in Lysate . It contains recombinant Human BAMBI, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Shipping

This ELISA Kit is shipped at ambient temperature.

Storage

Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA

BAMBI ELISA Kit, Human: Images

Human BAMBI/NMA ELISA Kit Standard Curve 88

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

BAMBI ELISA Kit, Human: Synonyms

NMA ELISA Kit, Human

BAMBI Background Information

BMP and activin membrane-bound inhibitor (BAMBI) is a transmembrane glycoprotein that is a pseudoreceptor of type 1 receptors. BAMBI structurally lacks intracellular serine/ threonine kinase domain but with an extracellular domain and a short cytoplasmic region that share sequence similarities with type 1 receptors, whose members have functions in signal transduction in various developing and pathological processes. BAMBI competes with the type 1 receptor, a receptor of the transforming growth factor-beta (TGF-beta), through functioning as negative regulators of TGF-beta by limiting the signaling range of the TGF-beta family during early embryogenesis. The expression of BAMBI can be induced by accumulated beta-catenin and BMP. The expression level of BAMBI was found aberrantly elevated in most colorectal and hepatocellular carcinomas relative to the corresponding non-cancerous tissues. It suggestes that beta-catenin and TGF-beta interfere growth arrest by inducing the expression of BAMBI, and this may contribute to colorectal and hepatocellular tumorigenesis.

Full Name

BMP and activin membrane-bound inhibitor

References

Sekiya T, et al. (2003) Identification of BMP and Activin Membrane-bound Inhibitor (BAMBI), an Inhibitor of Transforming Growth Factor- Signaling, as a Target of the -Catenin Pathway in Colorectal Tumor Cells. The Journal of Biological Chemistry. 279:6840-6.
Shi YG, et al. (2003) Mechanisms of TGF- Signaling from Cell Membrane to the Nucleus. Cell. 113(6): 685-700.
Wanninger J, et al. (2011) Adiponectin induces the transforming growth factor decoy receptor BAMBI in human hepatocytes. FEBS Lett. 585(9):1338-44.

BAMBI Matched ELISA Antibody Pair Set,Human
Assay Range(pg/ml):9.38-600
SEK10890

BAMBI and CHGA in Prion Diseases: Neuropathological Assessment and Potential Role as Disease Biomarkers
Author
López-Pérez, Ó;Bernal-Martín, M;Hernaiz, A;Llorens, F;Betancor, M;Otero, A;Toivonen, JM;Zaragoza, P;Zerr, I;Badiola, JJ;Bolea, R;Martín-Burriel, I;
Year
2020
Journal
Biomolecules
Application
Elisa
PubMed ID: 32370154FoldedExpand

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Sino Biological/BAMBI ELISA Kit, Human/1/KIT10890

Description

Related Products

Citations

BAMBI ELISA Kit, Human: General Information

BAMBI ELISA Kit, Human: Images

BAMBI ELISA Kit, Human: Synonyms

BAMBI Background Information

Citation List

If you couldn"t find the specific citation, you can: