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Sino Biological/IL-4 Protein, Human, Recombinant/1/11846-HNAE

Description
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Citations
Protein Expression Service

IL-4 Protein, Human, Recombinant: Product Information

Purity

> 92 % as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.05-0.2 ng/mL.

Protein Construction

A DNA sequence encoding the mature form of human IL4 isoform 1 (P05112-1) (His 25-Ser 153) was expressed and purified with an initial Met.

Accession#

P05112-1

Expressed Host

E. coli

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human IL4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15 kDa as predicted.

Formulation

Lyophilized from sterile 50mM Tris, 300mM NaCl, pH 7.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-4 Protein, Human, Recombinant: Images

Human IL4/IL-4/Interleukin-4 Protein 9861

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.05-0.2 ng/mL.

IL-4 Protein, Human, Recombinant: Synonyms

BCGF-1 Protein, Human; BCGF1 Protein, Human; BSF-1 Protein, Human; BSF1 Protein, Human; IL-4 Protein, Human; Interleukin-4 Protein, Human

IL-4 Background Information

Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.

Cancer Immunotherapy
Immune Checkpoint
Immunotherapy
Targeted Therapy

Full Name

interleukin 4

Related Pathways

Common G-Chain signaling pathway

References

Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9.
Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.

Human IL-4 Proteins

IL-4 Protein, Human, Recombinant, BiotinylatedGMP-11846-HNAE-B
IL-4 Protein, Human, RecombinantGMP-11846-HNAE

Mouse IL-4 Proteins

IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His Tag)51084-M08B
IL-4 Protein, Mouse, Recombinant51084-MNAE

Canine IL-4 Proteins

IL-4 Protein, Canine, Recombinant70021-DNAE

Molecular cloning, recombinant expression and characterization of GMCSF from the rhesus monkey, Macaca mulatta
Author
Tao, Z;Yang, H;Jia, D;Wan, L;Cheng, J;Lu, X;
Year
2013
Journal
Dev. Comp. Immunol.
Application
cell-based (culture)
PubMed ID: 23352624FoldedExpand
IL-1β from M2 macrophages promotes migration and invasion of ESCC cells enhancing epithelial-mesenchymal transition and activating NF-κB signaling pathway
Author
Zhou, J;Zheng, S;Liu, T;Liu, Q;Chen, Y;Tan, D;Ma, R;Lu, X;
Year
2018
Journal
J. Cell. Biochem.
PubMed ID: 29737564FoldedExpand
ROCK Inhibitor Y27632 Induced Morphological Shift and Enhanced Neurite Outgrowth-Promoting Property of Olfactory Ensheathing Cells via YAP-Dependent Up-Regulation of L1-CAM
Author
Li, Y;Huo, S;Fang, Y;Zou, T;Gu, X;Tao, Q;Xu, H;
Year
2018
Journal
Front. Cell. Neurosci.
Application
cell-based
PubMed ID: 30618636FoldedExpand
Urotensin II, urotensin-related peptide, and their receptor in aortic valve stenosis
Author
Khan, K;Albanese, I;Yu, B;Shalal, Y;Al-Kindi, H;Alaws, H;Tardif, J;Gourgas, O;Cerutti, M;Schwertani, A;
Year
2019
Journal
The Journal of Thoracic and Cardiovascular Surgery
Application
cell-based
FoldedExpand
Combination therapy with dendritic cell vaccine and programmed death ligand 1 immune checkpoint inhibitor for hepatocellular carcinoma in an orthotopic mouse model
Author
Teng, C;Wang, T;Wu, T;Lin, J;Shih, F;Shyu, W;Jeng, L;
Year
2020
Journal
Ther Adv Med Oncol
FoldedExpand

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Sino Biological/IL-4 Protein, Human, Recombinant/1/11846-HNAE

Description

Related Products

Citations

Protein Expression Service

IL-4 Protein, Human, Recombinant: Product Information

IL-4 Protein, Human, Recombinant: Images

IL-4 Protein, Human, Recombinant: Synonyms

IL-4 Background Information

Human IL-4 Proteins

Mouse IL-4 Proteins

Canine IL-4 Proteins

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