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Expressionsystems/pVL1392, pVL1393 Transfer Vectors/91-013/1 Ea

pVL1393TransferVector

pVL1392andpVL1393aretransfervectorsdesignedforhigh-levelexpressionofaclonedgenedrivenbythestrongAutographacalifornicamultiplenucleopolyhedrovirus(AcMNPV)polyhedrinpromoter(pPolh).

Description:

pVL1392andpVL1393arepUC8basedplasmidscontainingtheAcMNPVpolyhedrinlocuslackingthemajorityofthepolyhedrinopenreADIngframe.Amultiplecloningsite(MCS)sequencehasbeeninserted37nucleotidesdownstreamfromtheoriginalpolyhedrinATGstartcodon,whichhasbeenmutatedtoATT.

pVL1392andpVL1393shareidenticalsequences,withtheexceptionthattheyeachhavethesameMCSinopposingorientations.AstartandstopcodonmustbeprovidedwiththegenethatistobeclonedintotheMCS.Formaximalrecombinantproteinexpression,thedistancebetweenthecloningsiteandtheATGstartoftheinsertedgeneshouldbekepttoaminimum,andshouldnotexceed100nucleotides.

Specification:

MediaTypeESF921andESFAF
PlatformCompatibilityBestBac1.0andBestBac2.0
ShippingconditionAmbient
Storagecondition2-8°C,protectfromlight


RecommendedStorageCondition

2–8ºC
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