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Expressionsystems/Production Boost Additive/95-006-01/1 Ea

ProductionBoostAdditive1LBottle

ExpressionSystems’ProductionBoostAdditive(PBA)isanutrientboostdesignedtoincreaseproteinexpressionbysupportingtheincreasedneedsofinsectcellsinfectedbyrecombinantbaculovirus.PBAisaserum-free,animal-freeformulationthatcomplementsESF921andESFAFinsectcellculturemediaandextendsthelateandvery-lateexpressionperiodofproductionbymaintaininghighcultureviABIlity.

Description:

ExpressionSystems’ProductionBoostAdditive(PBA)isanutrient
boostdesignedtoincreaseproteinexpressionbysupportingthe
increasedneedsofinsectcellsinfectedbyrecombinantbaculovirus.
PBAisaserum-free,animal-freeformulationthatcomplementsESF921
andESFAFinsectcellculturemediaandextendsthelateandvery-late
expressionperiodofproductionbymaintaininghighcultureviability.
PBAisacocktailofnutrientsdesignedforsupplementationatarateof
1–10%at6–36hourspostinfection.

  • Serum-free,animal-freeformulationworksseamlesslywithESF921andESFAF
  • Extendscultureperiodbymaintainingcellviability
  • Increasesproteinexpression        
  • Availablein100mLor1Lbottles—inquireforcustompackaging
  • Instockforimmediateshipment

Fig.1      Fig.2

TniProcells(Fig.1)wereinfectedwitharecombinantbaculovirusatanMOIof1.PBAwasaddedat1%oftheculturevolume18hourspostinfection.Sf9cells(Fig.2)wereinfectedatanMOIof0.02.PBAwasaddedat1%or5%oftheculturevolume36hourspostinfection.Therecombinantproteinwassecretedintotheculturesupernatantandharvesteddaily.ProteinexpressionlevelsweredeterminedbyproteinspecificELISA.

Specification:

MediatypeSerum-free,animal-freeliquid,1X
ShippingconditionAmbient
Storagecondition2–8⁰C,protectfromlight
Use-bydateOneyearfromdateofmanufacture

RecommendedStorageCondition

2–8ºC
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