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ExpressionSystems’ProductionBoostAdditive(PBA)isanutrientboostdesignedtoincreaseproteinexpressionbysupportingtheincreasedneedsofinsectcellsinfectedbyrecombinantbaculovirus.PBAisaserum-free,animal-freeformulationthatcomplementsESF921andESFAFinsectcellculturemediaandextendsthelateandvery-lateexpressionperiodofproductionbymaintaininghighcultureviABIlity.
ExpressionSystems’ProductionBoostAdditive(PBA)isanutrient
boostdesignedtoincreaseproteinexpressionbysupportingthe
increasedneedsofinsectcellsinfectedbyrecombinantbaculovirus.
PBAisaserum-free,animal-freeformulationthatcomplementsESF921
andESFAFinsectcellculturemediaandextendsthelateandvery-late
expressionperiodofproductionbymaintaininghighcultureviability.
PBAisacocktailofnutrientsdesignedforsupplementationatarateof
1–10%at6–36hourspostinfection.
Fig.1
Fig.2
TniProcells(Fig.1)wereinfectedwitharecombinantbaculovirusatanMOIof1.PBAwasaddedat1%oftheculturevolume18hourspostinfection.Sf9cells(Fig.2)wereinfectedatanMOIof0.02.PBAwasaddedat1%or5%oftheculturevolume36hourspostinfection.Therecombinantproteinwassecretedintotheculturesupernatantandharvesteddaily.ProteinexpressionlevelsweredeterminedbyproteinspecificELISA.
| Mediatype | Serum-free,animal-freeliquid,1X |
| Shippingcondition | Ambient |
| Storagecondition | 2–8⁰C,protectfromlight |
| Use-bydate | Oneyearfromdateofmanufacture |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn