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Expressionsystems/ESF 921 Insect Cell Culture Medium, Protein Free/96-001-08/1 Ea

ESF921InsectCellCultureMedium,ProteinFree8LMediaTransferBag

ESF921™InsectCellCultureMediumisacompleteserum-free,protein-freemediumdevelopedforrobustcellgrowth,proteinproductionandbaculovirusvectorproductionforawiderangeofinsectcellsincludingSf9,Sf21,Tni(HighFive™),andDrosophilaS2.ESF921containsL-GlutamineandKolliphor®P188(PluronicF68).

Description:

WidelyrecognizedastheindustrystandardforBEVSapplications,ExpressionSystemsinsectcellculturemediaaredesignedformaximumvirusandproteinexpression.Thisserum-free,protein-freeinsectcellculturemediumprovidesunmatchedcellgrowthandexpressionforawiderangeofinsectcellsincludingSf9,Sf21,Tni(HighFiveTM)andDrosophilaS2.

  • Sf9celldensitiesof15x106cells/mlorgreater
  • Produceshightiterbaculovirusvectoryieldsof5E8to3E9infectiousunits/ml
  • Availablein1Lbottles,bulkpackagingandsingle-usebagsthateliminatetheneedtotransfermedia
  • Complete,ready-to-use,containsL-glutamine
  • Idealforscale-upmanufacturing
  • Workswithawidevarietyofbaculovirusandhostcellplatforms
  • Instockforimmediateshipment
  • Adaptedcellsavailable

SuperiorGrowthandReproducibility

ExpressionSystems’ESF921TMInsectCellCultureMediumisdesignedformaximumexpressionforapplicationssuchas;genetherapydrugsubstance,virus-likeparticles,virusvectorsandproteinsofinterest.ESF921TMiswidelyrecognizedastheindustrystandardforthebaculovirusandinsecthostcellsystem.ThelinegraphpresentsreproducIBLegrowthcurvesforSf9cellculturesinninedifferentlotsofESF921TMmediummanufacturedoveraperiodof12months.

Sf9cellsfromamid-logphaseculturewereseededinto*9differentlotsofESF921TMatadensityof0.75×10,6/ml.Culturesweresampleddailytodetermineviablecellcount.

Specification:

MediatypeReady-to-useserum-freeliquid,1X
PlatformcompatibilityFunctionswithBestBac™,Bacmid,Bac-to-Bac®,BacMam™,MultiBac™andotherBEVSplatforms
CelllinesSpodopterafrugiperda(Sf9,Sf21),Trichoplusiani(TniandHighFive™)andDrosophilamelanogaster(S2)
Use-bydateOneyearfromdateofmanufacture

RecommendedStorageCondition

2–8ºC
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