ESF921DeltaSeriesFormulationsarebasedonthepopularESF921mediumandareavailableinavarietyofaminoaciddeficientformatsusedforstructuralanalysisandproteinlabeling. Offtheshelfformulationsincludeallaminoaciddeficientandmethioninedeficient.Selectaminoaciddeficienciesmaybecustomordered.
Selenomethionineincorporationisastandardmethodfordeterminingthephasesinproteincrystallographybysingle-ormultiwavelengthanomalousdispersion.Tosupporttheeffortsofscientistsusingthismethodology,ExpressionSystemssuppliesaMethionineDeficientmediumformulation.Se-Metincorporationlevelsarereportedtobetypically75%(ProteinScience16(9):2023-9 · October2007)butcanrangefrom70-100%usingthismedium.
StructuralBIOLOGistsareincreasinglyutilizingNMRspectroscopytoobtaininformationaboutthestructureanddynamicsofproteinsandtheircomplexes.Thesimplestandmostcost-effectivebiosyntheticlabelingmethodforproteinsolid-stateNMRistouniformlylabelallcarbonandnitrogenatomswith13Cand15N.ExpressionSystemsstocksanallaminoaciddeficientvariantwithintheDeltaseriestoenablescientistsinterestedinaddingasourceofisotopically-labeledaminoacids.Customformulationsmayalsoberequestedspecifyingaformulationdeficientincarbonsourcesorspecificaminoacids.Customformulationsrequirea20Lminimumorderandaredeliveredinapproximately6weeks.
Mediatype | Serum-freeliquid,1X |
Platformcompatibility | FunctionswithBestBac™,Bacmid,Bac-to-Bac®,BacMam™,MultiBac™andotherBEVSplatforms |
Celllines | Spodopterafrugiperda(Sf9,Sf21),Trichoplusiani(TniandHighFive™),andDrosophiliamelanogaster(S2) |
Shippingcondition | Ambient |
Storagecondition | 2-8⁰C,protectfromlight |
Use-bydate | Oneyearfromdateofmanufacture |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn