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Expressionsystems/ESF 921 Delta Series/96-200/1 Ea

ESF921DeltaSeriesMethionineDeficient

ESF921DeltaSeriesFormulationsarebasedonthepopularESF921mediumandareavailableinavarietyofaminoaciddeficientformatsusedforstructuralanalysisandproteinlabeling. Offtheshelfformulationsincludeallaminoaciddeficientandmethioninedeficient.Selectaminoaciddeficienciesmaybecustomordered.

Description:

Selenomethionineincorporationisastandardmethodfordeterminingthephasesinproteincrystallographybysingle-ormultiwavelengthanomalousdispersion.Tosupporttheeffortsofscientistsusingthismethodology,ExpressionSystemssuppliesaMethionineDeficientmediumformulation.Se-Metincorporationlevelsarereportedtobetypically75%(ProteinScience16(9):2023-9 · October2007)butcanrangefrom70-100%usingthismedium.

StructuralBIOLOGistsareincreasinglyutilizingNMRspectroscopytoobtaininformationaboutthestructureanddynamicsofproteinsandtheircomplexes.Thesimplestandmostcost-effectivebiosyntheticlabelingmethodforproteinsolid-stateNMRistouniformlylabelallcarbonandnitrogenatomswith13Cand15N.ExpressionSystemsstocksanallaminoaciddeficientvariantwithintheDeltaseriestoenablescientistsinterestedinaddingasourceofisotopically-labeledaminoacids.Customformulationsmayalsoberequestedspecifyingaformulationdeficientincarbonsourcesorspecificaminoacids.Customformulationsrequirea20Lminimumorderandaredeliveredinapproximately6weeks.

Specification:

MediatypeSerum-freeliquid,1X
PlatformcompatibilityFunctionswithBestBac™,Bacmid,Bac-to-Bac®,BacMam™,MultiBac™andotherBEVSplatforms
CelllinesSpodopterafrugiperda(Sf9,Sf21),Trichoplusiani(TniandHighFive™),andDrosophiliamelanogaster(S2)
ShippingconditionAmbient
Storagecondition2-8⁰C,protectfromlight
Use-bydateOneyearfromdateofmanufacture

RecommendedStorageCondition

2–8ºC
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