ESF921™InsectCellCultureMediumisacompleteserum-free,protein-freemediumdevelopedforrobustcellgrowth,proteinproductionandbaculovirusvectorproductionforawiderangeofinsectcellsincludingSf9,Sf21,Tni(HighFive™),andDrosophilaS2.ESF921containsL-GlutamineandKolliphor®P188(PluronicF68).
WidelyrecognizedastheindustrystandardforBEVSapplications,ExpressionSystemsinsectcellculturemediaaredesignedformaximumvirusandproteinexpression.Thisserum-free,protein-freeinsectcellculturemediumprovidesunmatchedcellgrowthandexpressionforawiderangeofinsectcellsincludingSf9,Sf21,Tni(HighFiveTM)andDrosophilaS2.
ExpressionSystems’ESF921TMInsectCellCultureMediumisdesignedformaximumexpressionforapplicationssuchas;genetherapydrugsubstance,virus-likeparticles,virusvectorsandproteinsofinterest.ESF921TMiswidelyrecognizedastheindustrystandardforthebaculovirusandinsecthostcellsystem.ThelinegraphpresentsreproducIBLegrowthcurvesforSf9cellculturesinninedifferentlotsofESF921TMmediummanufacturedoveraperiodof12months.
Sf9cellsfromamid-logphaseculturewereseededinto*9differentlotsofESF921TMatadensityof0.75×10,6/ml.Culturesweresampleddailytodetermineviablecellcount.
Mediatype | Ready-to-useserum-freeliquid,1X |
Platformcompatibility | FunctionswithBestBac™,Bacmid,Bac-to-Bac®,BacMam™,MultiBac™andotherBEVSplatforms |
Celllines | Spodopterafrugiperda(Sf9,Sf21),Trichoplusiani(TniandHighFive™)andDrosophilamelanogaster(S2) |
Use-bydate | Oneyearfromdateofmanufacture |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn