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HumanZyme/VEGF165 (Vascular Endothelial Growth Factor 165)/HZ-1038/10 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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HumanZyme/VEGF165 (Vascular Endothelial Growth Factor 165)/HZ-1038/10 µg

ProductDescription:

  • Endotoxin-free
  • Animal-derivedproductfree
  • LyophilizedandCarrierFree(CF)
  • AuthenticGlycosylatedHomodimer

Xeno-freeVEGF165isexpressedinhuman293cellsasahomodimericglycoproteinwithanapparentmolecularmassof45kDa.TheE.coliexpressedproteinisamonomer-dimermixturewithamolecularmass18to34kDa.Thecytokineisproducedinahumancellexpressionsystemwithserumfree,chemicallydefinedmedia.VEGF,alsoknownasvascularpermeABIlityfactor(VPF)orvasculotropin,isahomodimeric34-45kDa,heparin-bindingglycoproteinwithpotentangiogenic,mitogenic,andvascularpermeabilityenhancingactivitiesspecificforendothelialcells.

TechnicalSpecifications:

  • Species:Human
  • Expression:HEK293CellExpressed
  • Activity:Typically≤15ng/mLEC50
  • Purity:>95%
  • Endotoxin:<1EU/µg
  • MolecularMass:45kDa,homodimer,glycosylated
  • Formulation:lxPBS

PurityConfirmation:

TheproteinwasresolvedbySDS-polyacrylamidegelelectrophoresisandthegelwasstainedwithCoomassieblue.

ActivityAssay:

Theactivitywasdeterminedbythedose-dependentstimulationoftheproliferationofHUVECcells(HumanUmbilicalVeinEndothelialCells).

ReconstitutionBuffer:

Brieflycentrifugethevialbeforeopening.Itisrecommendedtoreconstitutetheproteininsterile1xPBScontaining0.1%endotoxin-freerecombinantserumalbumin(HSA).

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