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HumanZyme/sRANK Ligand (soluble Receptor Activator of NFkB Ligand)/HZ-7231/500 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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HumanZyme/sRANK Ligand (soluble Receptor Activator of NFkB Ligand)/HZ-7231/500 µg

 ProductDescription:

RANKLandRANKaremembersoftheTNFsuperfamilyofligandsandreceptorsthatplayanimportantroleintheregulationofspecificimmunityandboneturnover.RANK(receptor)wasoriginallyidentifiedasadendriticcell-membraneprotein,which,byinteractingwithRANKL,augmentstheABIlityofdendriticcells.ThesedendriticcellsthenstimulatenaïveT-cellproliferationinamixedlymphocytereaction,promotethesurvivalofRANK+T-cells,andregulateT-cell-dependentimmuneresponse.RANKL,whichisexpressedinavarietyofcells,includingosteoblasts,fibroblasts,activatedT-cellsandbonemarrowstromalcells,isalsocapableofinteractingwithadecoyreceptorcalledOPG.BindingofsolubleOPGtosRANKLinhibitsosteoclastogenesisbyinterruptingthesignalingbetweenstromalcellsandosteoclasticProgenitorcells,therebyleADIngtoexcessaccumulationofboneandcartilage.HumanRANKLisreactiveonmurinecells.

TechnicalSpecifications:

  • Species:Human
  • Expression:E.coliCellExpressed
  • Activity:Typically10-25ng/mlED50
  • Purity:≥98%
  • Endotoxin:<1.0EU/μg
  • MolecularMass:20.0kDa
  • Formulation: 5mMSodiumPhosphate,pH7.6+75mMNaCl
  • CountryofOrigin:USA

PurityConfirmation:

ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.

ActivityAssay:

DeterminedbyitsabilitytoinduceNF-κBinRAW264.7cellsintheabsenceofanycross-linking.TheexpectedED50forthiseffectis10.0-25.0ng/ml.

AASequence:

MEKAMVDGSWLDLAKRSKLEAQPFAHLTIN
ATDIPSGSHKVSLSSWYHDRGWAKISNMTF
SNGKLIVNQDGFYYLYANICFRHHETSGDL
ATEYLQLMVYVTKTSIKIPSSHTLMKGGST
KYWSGNSEFHFYSINVGGFFKLRSGEEISI
EVSNPSLLDPDQDATYFGAFKVRDID

ReconstitutionBuffer:

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.

Storage:

Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.

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