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HumanZyme/BDNF (Brain-Derived Neurotrophic Factor)/HZ-7002/100 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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HumanZyme/BDNF (Brain-Derived Neurotrophic Factor)/HZ-7002/100 µg

ProductDescription:

BDNFisamemberoftheNGFfamilyofneurotrophicgrowthfactors.Likeothermembersofthisfamily,BDNFsupportsneuronproliferationandsurvival.BDNFcanbindtoalowaffinitycellsurfacereceptorcalledLNGFR,whichalsobindsotherneurotrophinssuchasNGF,NT-3andNT-4.However,BDNFmediatesitsneurotrophicpropertiesbysignalingthroughahighaffinitycellsurfacereceptorcalledgp145/trkB.BDNFisexpressedastheC-terminalportionofa247aminoacidpolypeptideprecursor,whichalsocontainsasignalsequenceof18aminoacidresiduesandapropeptideof110aminoacidresidues.RecombinantHumanBDNFisa27.0kDahomodimeroftwo120aminoacidsubunitslinkedbystrongnon-covalentinteractions.HumanandMouseBDNFsequencesareidentical.

TechnicalSpecifications:

  • Species:Human
  • Expression:E.coliCellExpressed
  • Activity:Typically0.5-1.0μg/mLED50
  • Purity:>98%
  • Endotoxin:<1.0EU/μg
  • MolecularMass:27.0kDa,homodimer
  • CountryofOrigin:USA

PurityConfirmation:

ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.

ActivityAssay:

DeterminedbyitsABIlitytostimulatetheproliferationofratC6cells.

AASequence:

MHSDPARRGELSVCDSISEWVTAADKKTAV
DMSGGTVTVLEKVPVSKGQLKQYFYETKCN
PMGYTKEGCRGIDKRHWNSQCRTTQSYVRA
LTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

ReconstitutionBuffer:

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.

Storage:

Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.

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