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HumanZyme/BMP-13 (Bone Morphogenetic Protein-13)/HZ-7203/10 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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HumanZyme/BMP-13 (Bone Morphogenetic Protein-13)/HZ-7203/10 µg

ProductDescription:

BMP-13isexpressedinhypertrophicchondrocytesduringembryonicdevelopmentoflongbones.ContinuedpostnatalexpressionofBMP-13inarticularcartilagesuggeststhatitplaysaregulatoryroleinthegrowthandmaintenanceofarticularcartilage.Adenovirus-mediatedBMP-13genetransfertorabbitbonemarrowstemcellshavebeenreportedtoaugmentperiostealrepairofosteochondraldefects.ThefunctionalformofBMP-13/CDMP-2isadisulfide-linkedhomodimeroftwo120aminoacidpolypeptidechains.This27.5kDaproteinisobtainedbyproteolyticprocessingofaBIOLOGicallyinactiveprecursorproteinof97.7kDa.RecombinantHumanBMP-13/CDMP-2isa27.0kDahomodimericdisulfide-linkedproteinconsistingoftwo120aminoacidpolypeptidechains.

TechnicalSpecifications:

  • Species:Human
  • Expression:E.coliCellExpressed
  • Activity:Typically2.0-3.0μg/mLED50
  • Purity:≥95%
  • Endotoxin:<1.0EU/μg
  • MolecularMass:27.0kDa
  • CountryofOrigin:USA

PurityConfirmation:

ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.

ActivityAssay:

DeterminedbyitsABIlitytoinducealkalinephosphataseproductionbyATDC-5cells.

AASequence:

TAFASRHGKRHGKKSRLRCSKKPLHVNFKE
LGWDDWIIAPLEYEAYHCEGVCDFPLRSHL
EPTNHAIIQTLMNSMDPGSTPPSCCVPTKL
TPISILYIDAGNNVVYKQYEDMVVESCGCR

ReconstitutionBuffer:

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.

Storage:

Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.

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