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HumanZyme/IGF-I (Insulin-like Growth Factor-I)/HZ-7045/100 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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HumanZyme/IGF-I (Insulin-like Growth Factor-I)/HZ-7045/100 µg

ProductDescription:

TheIGFsaremitogenic,polypeptidegrowthfactorsthatstimulatetheproliferationandsurvivalofvariouscelltypes,includingmuscle,bone,andcartilagetissueinvitro.IGFsarepredominantlyproducedbytheliver,althoughavarietyoftissuesproducetheIGFsatdistinctivetimes.TheIGFsbelongtotheInsulingenefamily,whichalsocontainsinsulinandrelaxin.TheIGFsaresimilartoinsulinbystructureandfunction,buthaveamuchhighergrowth-promotingactivitythaninsulin.IGF-IIexpressionisinfluencedbyplacentalactogen,whileIGF-Iexpressionisregulatedbygrowthhormone.BothIGF-IandIGF-IIsignalthroughthetyrosinekinasetypeIreceptor(IGF-IR),butIGF-IIcanalsosignalthroughtheIGF-II/Mannose-6-phosphatereceptor.MatureIGFsaregeneratedbyproteolyticprocessingofinactiveprecursorproteins,whichcontainN-terminalandC-terminalpropeptideregions.RecombinantHumanIGF-IandIGF-IIareglobularproteinscontaining70and67aminoacids,respectively,and3intra-moleculardisulfidebonds.

TechnicalSpecifications:

  • Species:Human
  • Expression:E.coliCellExpressed
  • Activity:Typically≤2.0ng/mLED50
  • Purity:>98%
  • Endotoxin:<1.0EU/μg
  • MolecularMass:7.6kDa
  • CountryofOrigin:USA

PurityConfirmation:

ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.

ActivityAssay:

DeterminedbyacellproliferationassayusingFDC-P1cells.

AASequence:

GPETLCGAELVDALQFVCGDRGFYFNKPTG
YGSSSRRAPQTGIVDECCFRSCDLRRLEMY
CAPLKPAKSA

ReconstitutionBuffer:

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.

Storage:

Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.

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